Abstract
A modification of the IBR-IPV virus-serum neutralization test (tissue culture tube test) was used in examinations of three groups of cattle: animals at AI centres; selected herds from infected districts; and pregnant cows and heifers from all over the country. In, respectively, 141 samples out of 1335, 215 out of 344, and 46 out of 7928, a virus-neutralizing effect was demonstrated. Within the three groups of samples, respectively, 2.8 %, 29.4 %, and 28.3 % of samples positive by the modified test were negative by the conventional test, even with undiluted serum. The findings gave strong evidence that all positive reactions were results of a preceding infection. All animals with a history of infection responded serologically when examined by the modified test, but still the distribution of the titers recorded in herd examinations indicates the desirability of a further improvement in the sensitivity of the test.
Keywords: IBR-IPV, infectious bovine rhinothraceitis, infectious postular vulvovaginitis, neutralization test, sensitivity
Sammendrag
En modificeret IBR-IPV-neutralisationstest baseret på vævskulturglas har været anvendt ved undersøgelse af 3 grupper af kvæg: dyr på tyrestationer, udvalgte besætninger fra inficerede områder og drægtige køer og kvier fra hele landet. Virus-neutralisation påvistes i henholdsvis 141 af 1335, 215 af 344 og 46 af 7928 undersøgte prøver. Af de positive prøver i grupperne var henholdsvis 2,8 %, 29,4 % og 28,3 % negative ved den konventionelle test, selv ved anvendelse af ufortyndet serum. Undersøgelserne indicerede, at alle positive resultater opnået med den modificerede test var signifikante som udtryk for en forudgående infektion med IBR-IPV-virus, og alle dyr, som havde vist klinisk sygdom, eller som måtte være blevet smittet, fandtes serologisk positive. Fordelingen af titrene for de fra besætningsunder-søgelserne positive prøver påpeger imidlertid ønskeligheden af en yderligere forbedret sensitivitet af testen.
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