Abstract
Experiments have been carried out with vaccination of pregnant mice against E. coli, followed by i.p. challenge of the offspring at one week of age.
With a septicaemic strain the results were highly significant, and the method is therefore recommendable for testing of vaccines against such strains of E. coli.
Results were less clear-cut with enteropathogenic strains of E. coli. However, with mortality rates of 40 to 45 % in baby mice born by non-vaccinated mothers and less than 15 % in baby mice born by vaccinated mothers, the difference in percentage mortality seems sufficient to warrant the use of the method also in the control of vaccines against enteropathogenic E. coli strains.
Keywords: baby mice, E. coli vaccine, potency-test
Sammendrag
Der er gennemført forsøg med vaccination af drægtige mus med E. coli vacciner og påfølgende i.p. challenge af afkommet i 7 dages alderen.
Ved forsøg med septikæmiske stammer fik man udtalt signifikante resultater, og metoden kan derfor anbefales til kontrol med vacciner mod septikæmiske stammer af E. coli.
Resultaterne var mindre udtalte ved forsøg med enteropatogene stammer af E. coli. Med en mortalitet på 40–45 % blandt museunger efter uvaccinerede mødre og under 15 % blandt museunger efter vaccinerede mødre synes forskellen i mortalitet tilstrækkelig til at betragte metoden anvendelig også i kontrollen med vacciner mod enteropatogene stammer af E. coli.
Full Text
The Full Text of this article is available as a PDF (723.9 KB).
References
- Dam A. Prophylaxis of Coli septicaemia in calves by vaccination of the dams. Serological and electrophoretical studies and calf-inoculation experiments. Proc. 4th Congr. Opatija: Cattle Diseases; 1968. pp. 247–253. [Google Scholar]
- Dam A. Preliminary experiments on vaccination and serum treatment in the prophylaxis of E. coli O149:K91:H10 infections in newborn piglets. Acta vet. scand. 1971;12:457–459. doi: 10.1186/BF03547747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dam A. The fate of E. coli organisms, strain A1, serotype 0149:K91: H10, in newborn piglets from sows immunized with an E. coli 0149 vaccine. Acta vet. scand. 1972;13:140–142. [PubMed] [Google Scholar]
- Huygelen C, Fasseaux P, Dobrescu Lucia. Immunization of mice against experimental Escherichia coli infection. Zbl. Vet.-Med. R. 1971;18:761–769. doi: 10.1111/j.1439-0450.1971.tb01653.x. [DOI] [PubMed] [Google Scholar]
- Smith, S. E. G.: The control of post-weaning E. coli enteritis of pigs using an inactivated adsorbed vaccine. 2nd Congr. Int. Pig vet. Soc, Hannover 1972.