Abstract
Investigated strains of Bacillus thuringiensis were all good producers of casein precipitating proteinases (CP-enzymes).
CP-enzymes produced by five strains of B. thuringiensis were serologically compared with enzymes produced by strains of B. cereus, B. licheniformis and B. subtilis. The technique used is a special enzymoserological procedure by which the proteolytic activity is neutralized by specific antisera. A close enzymoserological relationship was demonstrated between B. thuringiensis and B. cereus, while no interspecies cross-reactions occurred between enzymes of these species, and those of B. licheniformis and B. subtilis.
The possibility of undetected transference of B. thuringiensis from bacteriologically treated plant material to animals is discussed in relation to the high degree of similarity between B. cereus and B. thuringiensis.
Keywords: Bacillus thuringiensis, Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, enzymoserological examination, proteolytic enzymes
Sammendrag
Alle undersøkte stammer av Bacillus thuringiensis var gode produsenter av kaseinpresipiterende proteinaser (CP-enzymer).
CP-enzymer produsert av fire stammer av B. thuringiensis ble sammenlignet serologisk med enzymer produsert av B. cereus, B. licheniformis og B. subtilis. Den anvendte teknikk er en spesiell enzymoserologisk metode hvorved den proteolytiske aktivitet blir nøytralisert av spesifikke antisera. Det ble funnet et nært enzymoserologisk slektskap mellom B. thuringiensis og B. cereus, mens det ikke ble påvist kryssreaksjoner mellom enzymer fra disse arter på den ene side og enzymer fra B. licheniformis og B. subtilis på den annen.
Muligheten for uoppklart overføring av B. thuringiensis fra bakteriebehandlet plantemateriale til dyr er diskutert ut fra den høye grad av likhet som eksisterer mellom B. cereus og B. thuringiensis.
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