Abstract
Methods of collecting sputum and tracheobronchial washings are described.
Proteins in normal porcine tracheobronchial secretions are studied by immunochemical qualitative and quantitative methods. Among the immunoglobulins, IgA, IgG and IgM are identified. Immunoelectrophoretic analysis of sputum shows albumin, transferrin and in the α-region 2 components not found in serum. The predominant immunoglobulin in tracheobronchial secretions is IgA, which accounted for about 85 % of the total immunoglobulin concentration in sputum and 61 % of the immunoglobulin concentration in tracheobronchial washings.
Keywords: immunoglobulins, pigs, tracheobronchial secretions
Sammanfattning
Metoder att samla sputum och tracheobronchial spolvätska beskrivs. Proteiner i normala tracheobronchiala sekret hos svin studeras med immunokemiska kvalitativa och kvantitativa metoder. Bland immunoglobulinerna identifieras IgA, IgG och IgM. Immunoelektroforetisk analys av sputum visar albumin, transferrin och i α-regionen två komponenter vilka ej kan påvisas i serum. Det dominerande immunoglobulinet i tracheobronchiala sekret är IgA, som utgör cirka 85 % av den totala immunoglobulinkoncentrationen i sputum och cirka 61 % av totala immunoglobulinkoncentrationen i tracheobronchial spolvätska.
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Footnotes
Supported by the Swedish Council for Forestry and Agricultural Research.
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