Abstract
Chromic chloride was used for the coupling of chicken thyroid extract on human and chicken erythrocytes. With the coated cells thyroglobulin antibodies were determined in Obese Strain chicken sera. Ultracentrifugation of the sera prior to testing could be omitted. The method is more sensitive, less antigen requiring and less time-consuming than the previous tanned agglutination test.
Keywords: autoimmunity, thyroiditis, chicken, passive hemagglutination, chromic chloride coupling
Sammanfattning
Kromklorid användes för att fästa höns-sköldkörtelextrakt på humana och på hönserythrocyter. Med så preparerade celler bestämdes immunkroppar mot thyroglobulin i sera från höns av Obese-stam. Ultracentrifugering av sera för försöket var onödig. Metoden är känsligare och snabbare och antigenbehovet mindre jämfört med den tidigare tekniken där tanninbehandlade blodkroppar användes.
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