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. 1973 Jun 1;14(2):278–291. doi: 10.1186/BF03547446

Preparation Of Protein-Steroid Conjugates And Antisera Against 0Estradi0L-17β

Framstälining au protein-steroid konjugat och antisera mot 17β-estradiol

Kjell Martinsson 1,2,✉, Paul Lindberg 1,2, Elof D B Johansson 1,2
PMCID: PMC8559843  PMID: 4199483

Abstract

A conjugate of oestradiol-17β and bovine serum albumin (BSA) has been prepared by the mixed anhydride method. The conjugate was characterized by u.v.-analyses and immunoelectrophoresis, and the number of moles of oestradiol-17β per mole of BSA was found to be 25. The conjugate was used for immunization of two sheep and one rabbit in order to elict antisera against oestradiol-17β. Antibodies could be detected in all three animals after 5–16 weeks depending on the route of immunization. The antiserum from one sheep could be used in a radioimmunoassay system for oestradiol-17β in a dilution of 1:5000 and was found to cross-react with oestrone and oestradiol-17α to a lesser extent than another antiserum against oestradiol-17β.

Keywords: radioimmunoassay, oestrogens

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Footnotes

This study was supported by the Swedish Medical Research Council (B 72-17x-3495-01), the Ford Foundation, the Population Council, and the John M. Olin Foundation.

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