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. 1973 Mar 1;14(1):92–106. doi: 10.1186/BF03547413

The Generalized Shwartzman Reaction in Pigs Induced by Diet and Single Injection of Disintegrated Cells or Partially Purified Endotoxin from Escherichia Coli

Den generaliserte Shwartzmans reaksjon (GSR) hos gris fremkalt ved hjelp av diett og en enkelt injeksjon av knuste bakterier eller deluis renset endotoksin fremstilt av Escherichia coli

Jon Teige Jr 1,✉, Knut Nordstog 1, Morten Fjølstad 1, Inger Nafstad 1
PMCID: PMC8559851  PMID: 4580958

Abstract

Two experiments (Exps. I and II) were performed in pigs to test the effect of disintegrated cells and partially purified endotoxin derived from E. coli. The pigs were fed a diet supplemented with cod liver oil in one group, cod liver oil plus vitamin E in another group, and only commercial pig food in a third group. After one injection of disintegrated cells in Exp. I, lesions characteristic for generalized Shwartzman reaction (GSR), including hemorrhagic necrosis of the skin developed in the two groups given cod liver oil. A single case was also recorded among the control pigs after injection of a large dose. One injection of partially purified endotoxin in Exp. II gave almost similar results as in Exp. I. These two experiments show that pigs fed relatively high amounts of unsaturated fat are predisposed for the GSR and that vitamin E may, to a certain extent, protect against the development of the reaction.

Keywords: generalized Shwartzman reaction, pig, endotoxin, Escherichia coli, unsaturated fat, vitamin E

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References

  1. Baustad B, Nafstad I. Anemi hos voksende griser ved E-vitaminmangel. (Anemia in growing pigs with vitamin E-deficiency) Medlemsbl. norske Vet.-foren. 1968;20:82. [Google Scholar]
  2. De Navasquez S. Experimental tubular necrosis of the kidneys accompanied by liver changes due to dioxan poisoning. J. Hyg. (Lond.) 1935;35:540–548. doi: 10.1017/s0022172400032551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Di Luzio N R. Reticuloendothelial involvement in lipid metabolism. Ann. N. Y. Acad. Sci. 1960;88:244. doi: 10.1111/j.1749-6632.1960.tb20024.x. [DOI] [PubMed] [Google Scholar]
  4. Dodds W J, Miller K D. Dietary-induced atherosclerosis in miniature swine. Hematologic studies: Clotting factors and related hematologic values. Exp. molec. Path. 1968;8:263–301. doi: 10.1016/S0014-4800(68)80001-2. [DOI] [PubMed] [Google Scholar]
  5. Dubber A H C, Rifkind B, Gale M, McNicol G P, Douglas A S. The effect of fat feeding on fibrinolysis “Stypnen” time and platelet aggregation. J. Atheroscler. Res. 1967;7:225–235. doi: 10.1016/S0368-1319(67)80083-8. [DOI] [PubMed] [Google Scholar]
  6. Grant, C. A.: Morphological and aetiologieal studies of dietetic microangiopathy in pigs (“Mulberry heart”). Acta vet. scand. 1961, 2, Suppl. 3.
  7. Greig, H. B. W.: Inhibition of fibrinolysis by alimentary lipaemia. Lancet 1956, ii, 16–18. [DOI] [PubMed]
  8. Jarvis B W. Dietary lipids and coagulation. In vitro studies of coagulation mechanism in rats fed hyperlipemic diet. Arch. Path. 1967;83:364–369. [PubMed] [Google Scholar]
  9. Kaunitz H E, Malins D C, McKay D G. Studies of the generalized Shwartzman reaction produced by diet. II. Feeding of fractions of oxidized cod liver oil. J. exp. Med. 1962;115:1127–1136. doi: 10.1084/jem.115.6.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaunitz H E, Gauglitz E Y, Jr., McKay D G. Studies of the generalized Scwartzman reaction produced by diet. IV. Ethyl ester fractions of cod liver oil and dihydroxystearic acid. Metabolism. 1963;12:371–380. [PubMed] [Google Scholar]
  11. Lendrum, A. C., D. S. Fraser, W. Slidders & R. Henderson: Studies on character and staining of fibrin. J. clin. Path. 1962, 15, 401– 413. [DOI] [PMC free article] [PubMed]
  12. Mallory, F. B.: Pathological Technique. W. B. Saunders Co., Philadelphia 1942, p. 76.
  13. Marcus A J. The role of lipids in blood coagulation. Adv. Lipid Res. 1966;4:1–37. doi: 10.1016/B978-1-4831-9940-5.50008-9. [DOI] [PubMed] [Google Scholar]
  14. Margaretten W, Zunker H O, McKay D G. Production of the generalized Shwartzman reaction in pregnant rats by intravenous infusion of thrombin. Lab. Invest. 1964;13:552–559. [PubMed] [Google Scholar]
  15. McKay D G. A partial synthesis of the generalized Shwartzman reaction. Fed. Proc. 1963;22:1373–1379. [PubMed] [Google Scholar]
  16. McKay, D. G. & T. C. Wong: Studies of the generalized Shwartzman reaction produced by diet. I. Pathology. J. exp. Med. 1962 a, 115, 1117–1126. [DOI] [PMC free article] [PubMed]
  17. McKay, D. G. & T. C. Wong: Studies of generalized Shwartzman reaction produced by diet. III. Partial prevention by antibiotics. J. exp. Med. 1962 b, 115, 1137–1140. [DOI] [PMC free article] [PubMed]
  18. McKay D G, Margaretten W, Rothenberg J. Blockade of the reticuloendothelial system induced by dietary lipids. Lab. Invest. 1964;13:54–61. [PubMed] [Google Scholar]
  19. Mustard J F, Murphy E A. Effect of different dietary fats on blood coagulation, platelet economy and blood lipids. Brit. med. J. 1962;1:1651–1655. doi: 10.1136/bmj.1.5293.1651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Müller-Berghaus G. Pathology of Disseminated Intravascular Coagulation, Disseminated Intravascular Coagulation. Stuttgart-New York: F. K. Schattauer Verlag; 1969. pp. 45–61. [PubMed] [Google Scholar]
  21. Nafstad I. Light and electronmicroscopic studies on myocardial vascular injury in vitamin E-deficient pigs. Vet. Path. 1971;8:239–255. doi: 10.1177/030098587100800306. [DOI] [PubMed] [Google Scholar]
  22. Nafstad, I. & S. Tollersrud: The vitamin E-deficiency syndrome in pigs. I. Pathological changes. Acta vet. scand, 1970, 11, 452– 480. [DOI] [PMC free article] [PubMed]
  23. Neuwissen O J A Th, Hart H Ch, van Hemel-Rupert N S E. The influence of fats on blood coagulation and fibrinolysis. Thrombos. Diathes. haemorrh. (Stuttg.) 1968;19:267–278. [PubMed] [Google Scholar]
  24. Nordstoga K. Spontaneous bilateral renal cortical necrosis in animals. Path. Vet. 1967;4:233–244. doi: 10.1177/030098586700400303. [DOI] [PubMed] [Google Scholar]
  25. Nordstoga K, Fjølstad M. The generalized Shwartzman reaction and haemophilus infections in pigs. Path. Vet. 1967;4:245–253. doi: 10.1177/030098586700400304. [DOI] [PubMed] [Google Scholar]
  26. Nordstoga K, Fjölstad M. Porcine salmonellosis. II. Production of the generalized Shwartzman reaction by intravenous injections of disintegrated cells of Salmonella cholerae-suis. Acta vet. scand. 1970;11:370–379. doi: 10.1186/BF03547964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nordstoga K, Sparboe O, Stokkan M. Generalized Shwartzman reaction including extensive adrenal hemorrhage in a pregnant mare. Nord. Vet.-Med. 1968;20:330–337. [Google Scholar]
  28. Pearse, A. G. E.: Histochemistry. J. & A. Churchill, Ltd. London 1960, pp. 831–833.
  29. Renaud S, Allard C, Latour J G. Influence of the type of dietary saturated fatty acid on lipemia, coagulation and the production of thrombosis in the rat. J. Nutr. 1966;90:433–440. doi: 10.1093/jn/90.4.433. [DOI] [PubMed] [Google Scholar]
  30. Rowsell H C, Downie H G, Mustard J F. The experimental production of atherosclerosis in swine following the feeding of butter and margarine. Canad. med. Ass. J. 1958;79:647–654. [PMC free article] [PubMed] [Google Scholar]
  31. Teige, J. jr.: Unpublished data, 1971.
  32. Thomas L. Minnesota Heart Association. Minneapolis: Univ. Minn. Press.; 1952. The generalized Shwartzman reaction in rabbits infected with group H hemolytic streptococci. Rheumatic fever. A symposium; pp. 232–246. [Google Scholar]
  33. Thomas, L.: The Shwartzman phenomenon and other reactions produced by the endotoxins of Gram negative bacteria. Immun-pathology. 1st Int. Symp. ed. P. Grabar & P. Niescher. Benno Schwabe, Basel, Stuttgart 1959, 325–338.
  34. Thomas L, Good R A. Effect of cortisone on Shwartzman reaction, production of lesions resembling dermal and generalized Shwartzman reactions by single injection of bacterial toxin in cortisone-treated rabbits. J. exp. Med. 1952;95:409–428. doi: 10.1084/jem.95.4.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Tollersrud S, Nafstad I. The vitamin E-deficiency syndrome in pigs. II. Investigations on serum and tissue enzyme activity. Acta vet. scand. 1970;11:495–509. doi: 10.1186/BF03547949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Tompkins M J, Dayton S, Pearce M L. Effect of long-term feedings of various fats on whole blood clotting times in men. J. Lab. clin. Med. 1964;64:763–772. [PubMed] [Google Scholar]

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