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. 1973 Mar 1;14(1):44–56. doi: 10.1186/BF03547409

The Effects of Peptide-Precipitating Lignosulphonic Acids on the Proteolytic Activity of Pepsin in Vitro and on the Response of Pigs to an Ulcer Inducing Diet

Virkningen av peptidpresipiterende ligninsulfonsyrer på proteolytisk aktivitet av pepsin in vitro og på griser foret med en magesårfremkallende diett

Bjørn Næss 1,2,3,4,✉, Ola Westbye 1,2,3,4, Kjell Ivar Hildrum 1,2,3,4, Inger Nafstad 1,2,3,4
PMCID: PMC8559855  PMID: 4580955

Abstract

The inhibitory effect of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro has been studied. When an unfractionated lignosulphonic acid preparation was used, total inhibition of the activity of 5 µg crystalline pepsin was obtained by addition of about 15 mg of lignosulphonic acids.

The greatest inhibitory effect was seen with the high-molecular weight lignosulphonic acids, but an effect was also seen with middle-and low-molecular weight lignosulphonic acids.

A reduction in the inhibitory effect was seen when the experiments were performed in the presence of arginine. The inhibition mechanism is discussed in relation to the peptide-precipitating ability of the lignosulphonic acids.

No obvious protection against the appearance of stomach ulcers in four pigs receiving an ulcerogenic diet was seen.

Keywords: lignosulphonic acids, proteinase inhibition, pepsin, pig, ulcer inducing diet

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