Abstract
A number of biochemical and biological tests have been performed using reference strains of presently known mycoplasma species or sero-groups of bovine origin. The purpose of these investigations was partly to fulfil the requirements of “The Subcommittee on the Taxonomy of Mycoplasmatares” in describing new species of mycoplasmas, and partly to select methods which might be of value in daily diagnostic work. Concerning the latter point, the following tests are recommended for strains belonging to the digitonin resistant genus Acholeplasma: catabolism of galactose, xylose, aesculin and arbutin. In the genus Mycoplasma, which is digitonin sensitive, 5 tests are of special value: catabolism of glucose and arginine, phosphatase activity, formation of “film and spots”, and serum digestion.
Keywords: bovine mycoplasmas, biochemical characterization
Sammendrag
Referencestammer for de for tiden kendte bovine mykoplasmaarter eller serogrupper er undersøgt ved en række biokemiske og biologiske tests. Formålet med disse undersøgelser var dels at opfylde de krav, som er opstillet af „The Subcommittee on the Taxonomy of Mycoplasmatales“ ved beskrivelse af nye arter, og dels at udvælge metoder, som er af værdi i det daglige diagnostiske arbejde. I relation til det sidste punkt anbefales følgende undersøgelser for stammer tilhørende slægten Acholeplasma, som er digitonin resistent: Katabolisme af galaktose, xylose, aesculin og arbutin. For genus Mycoplasma, som er digitonin følsom, er følgende 5 prøver af særlig værdi: Katabolisme af glukose og arginin, phosphataseaktivitet, dannelse af “film and spots” og serumspaltning.
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