Figure 4.

Complete reversal of the function of miR-125b by modulation of target gene expression or treatment with anticancer drugs

(A and B) Flow cytometric analysis of apoptosis of NCI-H23 cells after co-transfection with miR-125b and MCL1 or BCL-w siRNA, or the respective controls, followed by Annexin V/propidium iodide staining. The percent differences in the rate of apoptosis for miR-125b-transfected cells relative to their respective controls (i.e., miR-control + the indicated siRNA) were quantified (n = 3).

(C) Cell viability of HCT116 cells after transfection with miR-125b or control, followed by treatment with the indicated drug. The results are the averages of four independent experiments.

Data are mean ± S.E.; ∗p < 0.01; ∗∗p < 0.05.