REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
RPMI-1640 Medium	Gibco	Cat #: 11,875-093
Dulbecco's Modified Eagle Medium (DMEM)	Gibco	Cat #: 11995-065
Fetal Bovine Serum	Gibco	Cat #: 16140-071
Penicillin-Streptomycin	Gibco	Cat #: 15140122
Lipofectamine RNAiMAX	ThermoFisher Scientific	Cat #: 13778030
Andy Fluor 647 Annexin V	ABP Biosciences	Cat #: A038
Annexin V Binding Buffer	BioLegend	Cat #: 422201
Propidium Iodide	MilliporeSigma	Cat #: P4170
TRIzol	ThermoFisher Scientific	Cat #: 15596026
mirVANA miR-125b mimic	Invitrogen	Cat #: 4464066-MC10148
mirVANA miRNA mimic negative control	Invitrogen	Cat #: 4464058
TW-37	Selleck Chemicals	Cat #: S1121
ABT-263 (Navitoclax)	Selleck Chemicals	Cat #: S1001
Doxorubicin hydrochloride	MilliporeSigma	Cat #: D1515
Camptothecin	MilliporeSigma	Cat #: C9911
5-Fluorouracil	MilliporeSigma	Cat #: F6627
Critical commercial assays
High-Capicity cDNA Reverse Transcription Kit	Applied Biosystems	Cat #: 4368814
Power SYBR Green PCR Master Mix	Applied Biosystems	Cat #: 4367659
CellTiter-Glo 2.0 Assay	Promega	Cat #: G9242
MycoAlert Plus Mycoplasma Detection Kit	Lonza	Cat #: LT07-701
Deposited data
The Cancer Genome Atlas (TCGA)	National Cancer Institute	Data Release 23.0
Experimental models: Cell lines
NCI-H23 cells	American Type Culture Collection (ATCC)	Cat #: CRL-5800
HCT116 cells	American Type Culture Collection (ATCC)	Cat #: CCL-247
Oligonucleotides
miR-125b miRNA mimic	GenePharma	double strand microRNA mimics (hsa-miR-125b-5p)
miRNA mimic negative control	GenePharma	microRNA mimics double strand negative control
MCL1 Forward Primer	Yale Keck Oligo Synthesis Resource	5’-TGCTTCGGAAACTGGACATCA-3’
MCL1 Reverse Primer	Yale Keck Oligo Synthesis Resource	5’-TAGCCACAAAGGCACCAAAAG-3’
BCL2 Forward Primer	Yale Keck Oligo Synthesis Resource	5′-TCGCCCTGTGGATGACTGA-3′
BCL2 Reverse Primer	Yale Keck Oligo Synthesis Resource	5′-CAGAGACAGCCAGGAGAAATCA-3′
BCL-w Forward Primer	Yale Keck Oligo Synthesis Resource	5’-GCGGAGTTCACAGCTCTATAC-3’
BCL-w Reverse Primer	Yale Keck Oligo Synthesis Resource	5’-AAAAGGCCCCTACAGTTACCA-3’
GAPDH Forward Primer	Yale Keck Oligo Synthesis Resource	5’-GGCCTCCAAGGAGTAAGACC-3’
GAPDH Reverse Primer	Yale Keck Oligo Synthesis Resource	5’-AGGGGTCTACATGGCAACTG-3’
Software and algorithms
MATLAB R2018a	MathWorks	https://www.mathworks.com/products/matlab.html
Computational model of the effect of miR-125b on the apoptosis pathway	supplemental information of this paper	Supplemental information of this paper
UCSC Xena Functional Genomics Explorer	Goldman et al., 2020	https://xena.ucsc.edu
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/