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. 2021 Oct 8;24(11):103241. doi: 10.1016/j.isci.2021.103241

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Hoil-1l^nzf∗/nzf; Sharpin^cpdm/cpdm mice show systemic inflammation and a distinctive immune cell composition

(A) Numbers of weaned and expected pups of the indicated genotypes from Hoil-1l^+/^nzf∗; Sharpin^+/cpdm crosses.

(B) Representative image of the gross appearance from 2-week-old mice of the indicated genotypes. Scale bar: 10 mm.

(C) Body weight of 4-week-old mice from the indicated genotypes.

(D) Representative images of the spleen of indicated genotype. Scale bar: 10 mm.

(E) Spleen weight of 4-week-old mice from the indicated genotypes.

(F) H&E staining, CD45R and CD3 immunostaining of spleen sections from 4-week-old mice of the indicated genotypes. Scale bar: 50 μm.

(G) Percentage of viable myeloid cells (CD11b⁺), normalized to the frequency of viable CD45⁺ cells, in the spleen from 4-week-old mice of the indicated genotypes.

(H) Percentage of neutrophils (CD11b^Hi Ly6G⁺) out of viable CD45⁺ cells in the spleen from 4-week-old mice of the indicated genotypes.

(I and J) Percentage of inflammatory monocytes (CD11c^- CD11b⁺ Ly6G⁻ Ly6C⁺) (I) and conventional monocytes (CD11c⁻ CD11b⁺ Ly6G⁻ Ly6C⁻) (J), out of viable CD45⁺ CD11b⁺ cells, in the spleen from 4-week-old mice of the indicated genotypes.

(K) Percentage of plasma cells (CD138⁺ CD28⁺), out of viable B220⁺ Lin⁻ cells, in the mesenteric lymph node (mLN) from 4-week-old mice of the indicated genotypes. The lineage (Lin) cocktail consists of TCRβ, DX5, CD11b, and CD23. Data are represented as mean ± SD. ANOVA, ∗∗p value≤0.01, ∗∗∗p value≤0.001, ∗∗∗∗p value ≤0.0001. (C and E) Hoil-1l^+/+; Sharpin^+/+; TnfrI^+/+ (n = 13 for C, n = 15 for E), Hoil-1l^{nzf∗/nzf∗}; Sharpin^+/⁺;TnfrI^+/+ (n = 10), Hoil-1l^+/+; Sharpin^cpdm/cpdm ;TnfrI^+/+ (n = 11 for C, n = 10 for E), Hoil-1l^+/nzf∗; Sharpin^cpdm/cpdm;TnfrI^+/+ (n = 25), Hoil-1l^{nzf∗/nzf∗};Sharpin^cpdm/cpdm;TnfrI^+/+ (n = 9 for C, n = 7 for E), Hoil-1l^{nzf∗/nzf∗}; Sharpin^cpdm/cpdm; TnfrI^−/− (n = 5) (G-K) Hoil-1l^+/+; Sharpin^+/+ (n = 8 for G-J, n = 6 for K), Hoil-1l^{nzf∗/nzf∗}; Sharpin^+/+ (n = 6 for G-J, n = 7 for K), Hoil-1l^+/+; Sharpin^cpdm/cpdm (n = 6), Hoil-1l^+/nzf∗; Sharpin^cpdm/cpdm (n = 9 for G-J, n = 8 for K), Hoil-1l^{nzf∗/nzf∗}; Sharpin^cpdm/cpdm (n = 4).