Abstract
The precipitation of peptide- and protein-lignosulphonic acid complexes can be demonstrated and studied in agar plates in the form of precipitation zones. The effects on the precipitates caused by variations in incubation temperature, incubation time, concentration of the reagents and in pH in the mixture of reagents, are described, and the nature of the peptide-lignosulphonic acid bond is discussed. The central lysis zones which appeared in some precipitation zones were found to be probably caused by excess of lignosulphonic acids. The possibility of developing the agar precipitation method described as a direct micro quantitative procedure for the determination of certain lignosulphonic acids in aqueous solution is suggested.
Sammendrag
Det har vist seg mulig å demonstrere og studere presipitering av peptider og proteiner med ligninsulfonsyrer i form av presipiteringssoner i agarplater. En har beskrevet virkningen på presipitatet av variasjon i inkuberingstemperatur, inkuberingstid, konsentrasjon av reagenter og pH i blandingen av reagenter, og en har diskutert bindingen i peptid-ligninsulfonsyre-komplekset. Lysissonene som ble utviklet sentralt i noen presipiteringssoner, er trolig forårsaket av overskudd av ligninsulfonsyrer. Muligheten for å utvikle den beskrevne agar-presipiteringsmetode som en direkte mikro-kvantitativ metode for å bestemme visse ligninsulfonsyrer i vandig løsning er antydet.
Full Text
The Full Text of this article is available as a PDF (1.9 MB).
Acknowledgement
The sulphite spent liquors used in this work were kindly supplied by A/S Tofte Cellulosefabrik, Hurum, Norway.
References
- Boriśek R, Stanic V. The precipitation of sulphite spent liquors by means of metal hydroxides: Theory and practice. Tappi. 1958;5:188–194. [Google Scholar]
- Crowle A J. Immunodiffusion. New York and London: Academic Press; 1961. pp. 23–26. [Google Scholar]
- Hall L. Sulfitavlutens tekniska användning. (Technical uses of sulphite spent liquor). Svensk Pappers-Tidn. 1956;59:716–720. [Google Scholar]
- Jantzen L. Personal communication. 1969. [Google Scholar]
- Jørgensen S E. Precipitation of proteins in waste water. Vatten. 1971;1:58–72. [Google Scholar]
- Pearl I A. Present status of chemical utilization of lignin. Forest Prod. J. 1957;12:427–432. [Google Scholar]
- Pearl I A. The Chemistry of Lignin. New York: Marcel Decker Inc.; 1967. [Google Scholar]
- Reich G. Gerbstoffe. (Tanning substances) In: F M, K S, editors. Verwertungsgebiete für Sulfitablauge. (Applications of sulphite spent liquor) Leipzig: VEB Deutscher Verlag für Grundstoffindustrie; 1967. pp. 280–288. [Google Scholar]
- Sandvik O. Studies on casein precipitating enzymes of aerobic and facultatively anaerobic bacteria. Oslo: Veterinary College of Norway; 1962. [Google Scholar]
- Sieber R. Die chemisch-technischen Untersuchungs-Methoden der Zellstoff- und Papier-Industrie. (The chemical and technical analysis methods of the chemical pulp and paper industry) 1951. pp. 327–328. [Google Scholar]
- Tønseth E J, Berridge H B. Removal of proteins from industrial waste waters. Effl. Water Treatm. J. 1968;8:124–128. [Google Scholar]