Abstract
The quantitative determination of fibrinogen in normal plasma and in cows with inflammatory conditions.
A rapid method for the quantitative determination of fibrinogen in bovine plasma is described.
The method was employed in the determination of normal values in a material consisting of 100 cows and 50 calves and young animals of various ages. The mean value of the groups of cows was approximately 0.550 g/100 ml. For young animals it was somewhat lower and for cows in the last month of gestation moderately higher than in the other groups.
The last part of the experiment involves the determination of the fibrinogen and γ-globulin levels in the plasma of 28 hospitalized cows with various inflammatory conditions. Group A in the material contained animals which were clinically cured and Group B animals that died or were killed.
Both groups showed a considerable increase in the fibrinogen level. In Group A the mean value fell back to approximately the normal range while in Group B it remained constantly elevated.
The sedimentation rate, SR, in human blood is primarily influenced by the fibrinogen content of the plasma. The SR in bovine blood is very low, and the test is therefore of little significance in diagnostic work. In conclusion, the possibility of using the fibrinogen determination in cattle for the same purpose as the SR in human blood is discussed.
Sammendrag
Det beskrives en hurtigmetode til kvantitativ fibrinogenbestemmelse i bovint plasma.
Metoden er anvendt for bestemmelse av normalverdier på et materiale bestående af 100 kuer og 50 kalver og ungdyr på forskjellige alderstrinn. Middelverdien hos voksne kyr lå omkring 0,550 g/100 ml. Helt unge kalver og ungdyr lå noe lavere og kyr i siste drektighetsmåned moderat høyere enn de øvrige grupper.
Siste del av forsøket omfatter bestemmelse av fibrinogen og γ-globulinnivå i plasma hos 28 hospitaliserte kyr med forskjellige infeksiøse prosesser. Gruppe A i materialet inneholdt dyr som klinisk ble restituert og gruppe B dyr som døde eller ble avlivet.
Begge grupper hadde en betydelig stigning i fibrinogenverdien. I gruppe A sank middelverdien til omkring normalområdet mens den i gruppe B stadig var forhøyet.
Senkningsreaksjon, SR, i humant blod er først og fremst påvirket av plasmaets fibrinogeninnhold. SR i bovint blod er meget liten og prøven derfor av liten betydning i diagnostikken. Sluttelig blir diskutert om fibrinogenbestemmelse hos storfe kan anvendes i samme øyemed som SR i humant blod.
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