Abstract
The leukocyte migration test for in vitro studies of delayed type hypersensitivity has recently been reviewed (Søborg & Ben-dixen 1967; Bendixen & Søborg 1969). Søborg & Bendixen applied the test to circulating leukocytes in man and thereby widely increased the potentialities of this test. They obtained high leukocyte yields with only moderate erythrocyte admixture by harvesting the supernatant plasma after sedimentation of the erythrocytes for 60 min. at 37°C in the normal gravitational field (1 × g)- Their procedure was unsuitable for the present investigation because bovine erythrocytes sediment so slowly. Sedimentation after clumping at the interphase of aqueous solutions of polymers, dextran and methylcellulose, in combination with metrizoic acid (Böyum 1968) was tried without success because the vast majority of the leukocytes sedimented together with the erythrocytes. Separation of leukocytes from erythrocytes could not be achieved by differential centrifugation.
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Acknowledgments
The State Veterinary Serum Laboratory, Dr. J. Berg Jørgensen, Dr. H. J. Bendixen and Dr. P. G0rtz, kindly serviced the complement fixation tests and the leukocyte counts. The authors are grateful to Mrs. Kirsten Poulsen for very skilled technical assistance.
References
- Bendixen G, Søborg M. A leukocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. A survey. Dan. med. Bull. 1969;16:1–6. [PubMed] [Google Scholar]
- Böyum A. Separation of leukocytes from blood and bone marrow. Scand. J. clin. Lab. Invest. 1968;21:1–109. [PubMed] [Google Scholar]
- Søborg M, Bendixen G. Human lymphocyte migration as a parameter of hypersensitivity. Acta med. scand. 1967;181:247–256. doi: 10.1111/j.0954-6820.1967.tb07255.x. [DOI] [PubMed] [Google Scholar]