TABLE 6.

Updated annotations add functional knowledge to genes required for optimal fitness during TB infection^a

Rv no.	Source annotation	New annotation	PubMed ID(s)	PDB ID(s)
Literature annotations
Rv1205	Hypothetical protein	Riboside monophosphate phosphoribohydrolase	25728768
Rv2018	Hypothetical protein	Probable antitoxin VapB/antigen	28066388, 23140854
Rv2272	Transmembrane protein	Probable gamma delta T-cell activator	23389928
Rv2525c	Tat pathway signal sequence	Probable peptidoglycan hydrolase	16952959, 25869294, 25260828
Rv2923c	Hypothetical protein	Probable osmotically induced bacterial protein C (OsmC, a probable peroxide reductase)	22088319
Rv3632	Membrane protein	Putative flippase	21030587
Rv3763	Lipoprotein LpqH	Adhesin/antigen LpqH	16098710, 12594264
Rv3788	Hypothetical protein	Probable secondary channel binding factor of RNA polymerase	22194445
Literature and structural inference
Rv0191	Integral membrane protein	Putative efflux pump	25690361, 12520088, 22132058	5c65, 4gby
Rv1433	Exported protein	Probable l,d-transpeptidase LdtMt3	24041897	3tur, 3vae, 4jmn
Rv1769	Hypothetical protein	T-cell antigen/putative aldehyde-lyase	26853625, 15102765	4v15
Rv3722c	Hypothetical protein	Probable serine hydrolase, probable aspartate transaminase		3ppl
Structural inference
Rv0047c	Hypothetical protein	Putative transcriptional regulator		1yg2a, 3l9f
Rv0259c	Hypothetical protein	Putative lyase		2jh3, 4ccs
Rv0323c	Hypothetical protein	Putative hydrolyase/putative linear amide hydrolase		1q7t, 5cgz
Rv0449c	Hypothetical protein	Putative oxidoreductase		2ive, 1sez, 3nks, 3i6d, 3lov
Rv0767c	Hypothetical protein	Putative transcription factor		3mnl
Rv1085c	Hemolysin-like protein	Putative membrane transporter receptor protein		5b2nA, 4xtlA
Rv2052c	Hypothetical protein	Putative endodeoxyribonuclease		3igh
Rv2160A	Hypothetical protein	Putative transcription factor		2hyj
Rv3226c	Hypothetical protein	Putative peptidase		2icu
Rv3433c	Hypothetical protein	Putative hydrolyase/putative isomerase		2ax3
Rv3719	Hypothetical protein	Putative amide-bond oxidoreductase		3dq0, 2exr, 4o95

^aSource annotation is the annotation listed by Bellerose et al. (83) and new annotation derived from the current project. Protein Data Bank identifiers (PDB ID) of the protein structures matching H37Rv protein models are listed for structure-based annotations. PubMed IDs are listed for the papers from which functional annotations were manually curated.