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(a) Parameters for the binding of CEF to BlaC [see also Fig. 3 ▸ and equation (4)]. The concentration E 0 of all subunits in the BlaC platelet crystal form is 15.5 mM. Only subunits B and D bind substrate. C 0,CEF is the mixed-in substrate concentration [see equation (3)].
| E 0 (mM) | C 0,CEF (mM) | k on (M −1 s−1) |
|---|---|---|
| 7.8 | 150 | 3.2 |
