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. 2021 Sep 23;40(44):6235–6247. doi: 10.1038/s41388-021-02017-8

Fig. 3. Loss of ISGylation is associated with decreased Akt phosphorylation.

Fig. 3

A RPPA analysis performed in WT and the different ISGylation CRISPR clones, treated with EGF 10 ng/ml for 0, 10, 30 and 60 min. Heatmap displays the Log2 of intensity normalised to WT at the different time points. Arrow indicates pAkt data. Averaged, n = 3 B Bar graph of pAkt intensities obtained in the RPPA. Values displayed are the mean ± S.E.M. n = 3. C Confirmation of RPPA data by WB analysis. Cells were treated with EGF 10 ng/ml for 10 min, WB and incubated with indicated antibodies.