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. 2021 Nov 3;6(12):3315–3317. doi: 10.1080/23802359.2021.1915197

Characterization of the complete mitochondrial genome of Dioszegia changbaiensis (Tremellales: Bulleribasidiaceae) with phylogenetic implications

Maoling Tan a, Qiangfeng Wang b,✉
PMCID: PMC8567934  PMID: 34746399

Abstract

In this study, the complete mitochondrial genome of Dioszegia changbaiensis we sequenced and assembled by the next-generation sequencing. The complete mitochondrial genome of Dioszegia changbaiensis contained 22 protein-coding genes (PCG), two ribosomal RNA (rRNA) genes, and 22 transfer RNA (tRNA) genes. The total length of the Dioszegia changbaiensis mitochondrial genome is 34,853 bp, and the GC content of the mitochondrial genome is 41.88%. Phylogenetic analysis based on a combined mitochondrial gene dataset indicated that the mitochondrial genome of Dioszegia changbaiensis exhibited a close relationship with that of Hannaella oryzae.

Keywords: Yeast, mitochondrial genome, phylogenetic analysis, molecular marker

The genus Dioszegia was described by Zsolt (1957). Since then, dozens of species have been described in the genus (Bai et al. 2002; Connell et al. 2010; Trochine et al. 2017). Species from the genus Dioszegia are distributed in a variety of ecological environments, such as on leaf surfaces, in plant roots and also in soil (Wang et al. 2003; Wang et al. 2008; Takashima et al. 2011). Dioszegia species was once moved to the genus Cryptococcus according to morphology (Takashima et al. 2011). With the progress of molecular techniques, the genus Dioszegia was separated from other genera as an independent genus (Takashima et al. 2001). Mitochondrial genomes have been widely used in the phylogeny of Basidiomycete species (Li, He, et al. 2020; Wang, Song, et al. 2020). The mitochondrial genome of D. changbaiensis reported here will promote the understanding of taxonomy and genetics of the Dioszegia genus.

The specimen (Dioszegia changbaiensis) was collected from Sichuan, China (102.53E; 31.25 N), and was stored in Culture Collection Center of Chengdu University (No. Dsp_na07). We sequenced and de novo assembled the complete mitochondrial genome of Dioszegia changbaiensis according to previous described methods (Li, Liao, et al. 2018; Li, Xiang, et al. 2019; Wang, Song, et al. 2020). Briefly, the total genomic DNA of Dioszegia changbaiensis was extracted using a Fungal DNA Kit (D3390-00, Omega Bio-Tek, Norcross, GA). And then we purified the genomic DNA using a Gel Extraction Kit (Omega Bio-Tek, Norcross, GA). The purified DNA was stored in Chengdu University (No. DNA_Dsp_na07). We constructed sequencing libraries of Dioszegia changbaiensis using a NEBNext® Ultra™ II DNA Library Prep Kit (NEB, Beijing, China). We conducted whole genomic sequencing (WGS) of Dioszegia changbaiensis using the Illumina HiSeq 2500 Platform (Illumina, SanDiego, CA). The mitochondrial genome of Dioszegia changbaiensis was de novo assembled using SPAdes version 3.9.0 (Bankevich et al. 2012; Li, Ren, et al. 2020). The obtained mitochondrial genome of Dioszegia changbaiensis was annotated according to previous described methods (Li, Chen, et al. 2018; Li, Wang, et al. 2018; Wang, Jia, et al. 2020; Ye et al. 2020).

The complete mitochondrial genome of Dioszegia changbaiensis is 34,853 bp in length. The base compositions of the Dioszegia changbaiensis mitochondrial genome were as follows: A (28.49%), T (29.63%), G (20.27%), and C (21.61%). The complete mitochondrial genome of Dioszegia changbaiensis contains 22 protein-coding genes (PCGs), two ribosomal RNA genes (rns and rnl), and 22 transfer RNA (tRNA) genes. To investigate the phylogenetic status of the mitogenome of Dioszegia changbaiensis in Basidiomycota, we constructed a phylogenetic tree for 18 Basidiomycete species. Rhizopogon salebrosus from the Boletales order was set as outgroup (Li, Ren, et al. 2019a). The phylogenetic tree was constructed using the Bayesian analysis (BI) method based on the combined 14 core PCGs according to previous described methods (Li et al. 2019a, 2019b; Li, Yang, et al. 2020). As shown in the phylogenetic tree (Figure 1), the mitochondrial genome of Dioszegia changbaiensis exhibited a close relationship with that of Hannaella oryzae (MH732752)

Figure 1.

Figure 1.

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Bayesian phylogenetic analysis of 18 species based on the combined 14 core protein-coding genes. Accession numbers of mitochondrial sequences used in the phylogenetic analysis are listed in brackets after species.

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Funding Statement

The study was supported by the Science and Technology support program of Sichuan [2019YFH0116].

Disclosure statement

The authors have declared that no competing interests exist.

Data availability statement

This mitogenome of Dioszegia changbaiensis was submitted to GenBank under the accession number of MT755637 (https://www.ncbi.nlm.nih.gov/nuccore/MT755637).

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

This mitogenome of Dioszegia changbaiensis was submitted to GenBank under the accession number of MT755637 (https://www.ncbi.nlm.nih.gov/nuccore/MT755637).

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