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. 2021 Oct 14;148(20):dev199731. doi: 10.1242/dev.199731

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Identification of Sec15 as a RASSF8 partner. (A) Schematic diagram of the RASSF8 and Sec15 proteins. The two-hybrid bait and prey, and the constructs used in the co-immunoprecipitation experiments are also shown [RASSF8: N, (amino acids) 1-120; C1, 121-607; C2, 350-607; C3, 490-607; Sec15: N1, 1-134; N2, 1-225; C1, 58-766; C2, 130-766]. (B-F) Co-immunoprecipitation experiment in S2 cells overexpressing the indicated constructs. (B) RASSF8-HA binds specifically to Sec15-Myc but not to Sec5-Myc. (C,D) Amino acids 350-490 of RASSF8 are necessary for Sec15 binding. (E,F) Amino acids 135-225 of Sec15 are required for its interaction with RASSF8.