Skip to main content
. 2021 Oct 14;148(20):dev199731. doi: 10.1242/dev.199731

Fig. 4.

Fig. 4.

Rab11 and Sec15, but not Sec5, are mislocalised in RASSF8 mutant clones. (A-D″) Confocal micrographs of pupal wing discs and pupal retinas at 26 h APF bearing sec15 (A-A″) or RASSF8 (B-D″) mutant clones generated using hsFLP (wing clones) or eyFLP (retinal clones) and stained as indicated. White dotted lines indicate the clone boundaries. (A-A″) Accumulation of Rab11 in sec15 mutant clones marked by the absence of RFP in the pupal wing. (B-B″) Sec5 staining is not affected in RASSF8 mutant pupal wing clones marked by the absence of RFP. The horizontal band of elevated Sec5 intensity in the mutant clones corresponds to a wing vein. In A-B″, the traces show the intensity profiles at the straight white dotted lines in the merged image using Fiji. (C-D″) Accumulation of Sec15 (C-C″) and Rab11 (D-D″) in RASSF8 mutant clones marked by the absence of GFP in pupal retinas. Scale bars: 10 μm. (E,F) Co-immunoprecipitation experiments in S2 cells overexpressing the indicated constructs. (E) Baz associates with RASSF8 but not with ASPP. (F) ASPP co-immunoprecipitates Baz only in the presence of RASSF8.