Abstract
Background
Intraoperative Cerenkov luminescence imaging (CLI) is a novel technique to assess surgical margins in patients undergoing nerve sparing radical prostatectomy (RP). Here, we analyze the efficacy of a 550-nm optical short-pass filter (OF) to improve its performance.
Methods
In this prospective single-center feasibility study ten patients with prostate cancer (PC) were included between December 2019 and April 2020, including three patients without tracer injection as a control group. After preoperative injection of 68-Ga-prostate-specific membrane antigen (PSMA)-11 followed by RP, CLI of the excised prostate and the incised index lesion was performed to visualize the primary tumor lesion. We compared the findings on intraoperative CLI to postoperative histopathology. Furthermore, CLI-intensities determined as tumor to background ratio (TBR) and contrast to noise ratio (CNR) were measured.
Results
Histopathology proved positive surgical margins (PSM) in 3 patients with corresponding findings in CLI. After magnetic resonance imaging (MRI)-informed incision above the index lesion 2 out of 3 prostates demonstrated elevated CLI signals with histopathological confirmation of PC cells. The use of the OF enabled a significant reduction of the area of the regions of interest from a median of 1.80 to 0.15 cm2 (reduction by 85%, P=0.005) leading to increased specificity. Signals due to PSMs were not suppressed by the 550-nm OF. The median TBR was reduced from 3.33 to 2.10. In all three patients of the control group elevated CLI intensities were detected at locations with diathermal energy deposition during surgery. After application of the 550-nm OF these were almost totally suppressed with a TBR of 1.10. Measurements of Cerenkov luminescence intensity with the 550-nm OF showed a significant Pearson’s correlation of 0.82 between PSM and the elevated TBR (P=0.003) and a significant Pearson’s correlation of 0.66 between PSM and elevated CNR (P=0.04). Measurements without the OF did not correlate significantly.
Conclusions
Intraoperative 68-Ga-PSMA CLI in PC is a tool that warrants further investigation to visualize PSM especially in intermediate and high-risk PC. Intraoperative CLI benefits from usage of a 550-nm OF to reduce false-positive signals.
Keywords: Prostate cancer (PC), radical prostatectomy (RP), Cerenkov luminescence imaging (CLI), margin assessment, 68-Ga-PSMA-11
Introduction
Radical prostatectomy (RP) is one of the primary treatment options for men with localized or locally advanced prostate cancer (PC) and aims at complete tumor resection without positive surgical margins (PSM) (1). Preoperative magnetic resonance imaging (MRI) improves knowledge of the localization of PC before RP (2,3). However, Gandaglia et al. recently demonstrated that both MRI and prostate sampling using systematic biopsy cores are needed to predict adverse pathology accurately (4). This is of relevance when planning patient-tailored preservation of the neurovascular bundles without increased risk and numbers of PSM (5,6). In addition to preoperative MRI for local staging and nomograms for the prediction of extraprostatic extension, the use of intraoperative frozen section analysis (IFS) can help the surgeon to preserve functional structures like the neurovascular bundles (1,6-8). However, besides resource consumption, there is also some conflicting evidence, as studies demonstrated high false-negative rates of IFS, potentially resulting in unjustified nerve-sparing surgery (7,9). Various pre- and intraoperative methods are currently part of scientific research with the aim of reducing or avoiding PSM as incomplete removal of aggressive cancer tissue during RP is associated with poorer patient outcomes (10).
Molecular imaging with modern radiopharmaceuticals that target the prostate-specific membrane antigen (PSMA) can be used for highly specific oncological diagnostic imaging, including initial staging of PC (11-14). PSMA positron emission tomography (PSMA-PET) is an imaging technique that targets PSMA on PC cells, mostly with 68Ga-labelled and 18F-labelled PET agents (15). In men with biochemical recurrence, PSMA-PET imaging has become the imaging modality of choice if the results will influence subsequent treatment decisions (16).
In recent trials, PSMA-PET demonstrated promising detection rates of lymph node metastases and high positive predictive values for initial staging of PC (17-20). A recently published randomized multicentre study proved a lower sensitivity and specificity for conventional primary staging with computed tomography (CT) and bone scan as compared to 68-Ga-PSMA/PET/CT (21). Maurer et al. described the advantage of PSMA-radio guided pelvic lymph node dissection, visualising even small lymph node metastasis (22). Consequently, there is a wish to be able to accurately detect malignant areas in real-time during RP to ensure that all PC is completely removed.
PET imaging agents also emit optical photons via a phenomenon called Cerenkov luminescence, enabling optical molecular imaging in the form of Cerenkov luminescence imaging (CLI) (23). Both techniques—CLI and PET—measure photons: PET measures gamma annihilation photons, and CLI measures Cerenkov photons (24). Cerenkov photons are emitted by a charged particle (positron or electron) when travelling through a dielectric medium at a faster speed than the velocity of light in that medium. Although Cerenkov luminescence has a broad wavelength spectrum, it predominantly comprises ultraviolet and blue light. These short wavelengths are highly attenuated in biological tissue. Therefore, CLI is limited to detection of signals emitted in superficial tissue layers (approximately 3 mm). In contrast to PET, CLI is unable to detect photons emitted by deeper located tissues or tumors (25,26). Intraoperative imaging with CLI is promising since it allows to evaluate the entire surface of the prostate instead of only specific IFS slices (27,28). Besides the good agreement between CLI and histopathology, there is also evidence of false-positive signals mainly at the bladder neck (27). Artificial signals caused by longer wavelengths can make intraoperative assessment more difficult and even lead to unnecessary resections of healthy tissue.
With regard to CLI, only few studies with small patient populations have been published so far. To our knowledge there is no published data available regarding CLI in PC investigating the benefit of an additional optical short-pass filter (OF). The main purpose of the present feasibility study was to improve the CLI efficiency through a 550-nm OF in order to filter out potential interfering signals and thus to improve detection of signals caused by carcinoma cells on the surface. The secondary objective was evaluation of potential interfering factors in relation to the intraoperative application of CLI and the different ratios for CLI assessment: Tumor to background ratio (TBR) and contrast to noise ratio (CNR). We present the following article in accordance with the STROBE reporting checklist (available at http://dx.doi.org/10.21037/tau-20-1141).
Methods
Patient recruitment and patient preparation on day of surgery
Patients for primary treatment with histologically confirmed intermediate and high-risk PC on systematic and/or MRI-targeted prostate biopsy were recruited at one study center between December 2019 and April 2020 after discussion in the local multidisciplinary tumor board. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013) and after written informed consent of the patients. The study was approved by the institutional ethics board of the University Duisburg-Essen (approval number 19-8749-BO). The study has been registered at German Clinical Trials register (DRKS-ID: DRKS00020942) and is recruiting patients at the time of submission. Only patients with intraoperative incision of the prostate gland above the index lesion were included. Exclusion criteria were previous prostate surgery or radiotherapy, known distant metastases in conventional staging according to the actual European Association of Urology (EAU) guidelines, and contraindications to surgery (1).
On the day of surgery, 68-Ga-PSMA-11 (1.8–2.2 MBq per kg/body weight) was injected intravenously in patients scheduled to undergo RP (29). Forty-five to 60 minutes after tracer injection, a PET/CT scan was performed and assessed by experienced nuclear medicine physicians. In case of high-volume metastatic disease on PET/CT, same-day surgery would have been cancelled.
RP and intraoperative CLI
Seven patients with preoperative 68-Ga-PSMA administration, intraoperative CLI as well as incision of the index lesion were included in the present feasibility study. The localization of the index lesion in the prostate was based on preoperative MRI and MRI-targeted biopsy. Final RP histopathology proved the accuracy of the localisation. After PET/CT, RP was performed by two surgeons ahead of extended pelvic lymph node dissection (ePLND) to minimize signal intensity (SI) reduction from radiotracer decay in the time between 68-Ga-PSMA injection and CLI. A urinary catheter was inserted in the operating room (OR) to drain the urine, minimizing urinary contamination of the prostate. The excised prostate specimen was immediately retrieved from the abdomen, wiped to clear blood and fluids, positioned in a specimen tray and then imaged in the LightPath® CLI system (Lightpoint Medical Ltd., Chesham, UK). The LightPath® system captured both a Cerenkov image and a photographic image of the specimen in a light-tight imaging chamber and was positioned in the OR to enable real-time image analysis by the surgeon. Images were acquired with a total acquisition time of 300 seconds and 8×8 pixel binning (30). Each examination was repeated with a 550-nm OF to ensure that the specimen was in the identical position. The prostate gland was imaged in different positions, and a total of two or three images were necessary to capture the entire surface area of the prostate. After the intact prostate was examined, the prostate gland was incised in the area of the index lesion on MRI and subsequently imaged in CLI to visualise the primary tumor. The incised specimens are imaged without the 550-nm OF. Upon CLI completion, the RP specimen was sent for postoperative histopathological examination. Due to the design of this feasibility study and limited evidence on the performance characteristics of CLI, the surgical course remained unaffected by the CLI results and no further tissue was resected if positive margins were suspected on CLI.
Image analysis
The CLI images were analysed postoperatively in a controlled and standardized analysis environment using PMOD (PMOD Version 3.204, PMOD Technologies LLC). Measurements of the mean radiance (photons/s/cm2/sr) were performed by manually drawing regions of interest (ROIs) on the CLI. ROIs were selected in areas showing increased SI and in areas with no increased SI (tissue background). Furthermore, TBRs as well as CNRs of the mean values were calculated. The following formulas have been used for the respective calculation: TBR = mean signal/mean background, CNR = (mean signal – mean background)/background standard deviation (31). Tumor margin assessment on CLI was performed by analysing elevated signals on the surface of the intact prostate. For background measurements a standardized ROI with an area of 0.8 cm2 was used. This standardized ROI was applied to each CLI image examined in this study. The area of the ROIs with elevated CLI intensities was measured with and without application of a 550-nm OF and is stated in cm2. In case of missing CLI signals with the 550-nm OF, the ROI of the measurement without 550-nm OF was transposed and used to determine the CLI intensity. In this case the measured area was not statistically evaluated. It should also be noted that the background signal of the prostate is inhomogeneous. Thus, in the calculated ratios, some negative results can occur if the signal of one ROI is lower than that of the predefined background. False positive signals have been defined as a TBR >1 without histopathologic prove of cancer cells in that area.
Histopathology
Histopathologic evaluation was performed adherent to the current German S3-guidelines for PC (32). RPs were evaluated by an expert uro-pathologists with 12 years of experience in genitourinary-pathology (H Reis) and typical PC metrics were reported including Gleason grading in its 2014 revision (33). Quarters have been digitally reconstructed to whole mounts to be able to compare them with the CLI images.
Statistical analysis
Numerical variables were summarized with median values and interquartile ranges (IQRs), categorical variables with proportions (%). To compare medians of non-parametric data, the Mann-Whitney U test was used for two groups. Pearson’s correlation coefficient was used to correlate PSM with elevated CLI intensities (significance set at P<0.05). Statistical analysis was performed with IBM SPSS® Statistics version 26 (IBM, Armonk, USA).
Results
Patients’ demographic and histopathological data are given in Table 1. Histopathology proved PSM in three patients with corresponding findings in CLI. Of note, in the seven patients undergoing 68-Ga-PSMA/PET-CT before surgery PET-avidity and multiparametric MRI (mpMRI) correlated with regards to the index lesion.
Table 1. Patient demographics, imaging and histopathologic characteristics.
| Variables | Study group | Control group |
|---|---|---|
| Patients, N | 7 | 3 |
| Median age, years [IQR] | 66 [59; 69] | 64 [55; 64] |
| Median PSA, ng/mL (IQR) | 12.0 (5.6; 16.0) | 9.5 (6.4; 9.5) |
| Median weight, kg [IQR] | 88 [75; 110] | 85 [72; 85] |
| Median tracer activity, MBq [IQR] | 127 [116; 179] | – |
| Median SUV of the prostate lesion, max. value [IQR] | 8.4 (5.7; 15.6) | – |
| Median activity at PET, kBq/mL (IQR) | 9.38 (5.17; 13.85) | – |
| Median activity at CLI, kBq/mL (IQR) | 0.38 (0.34; 0.89) | – |
| Median time to CLI, minutes [IQR] | 316 [283; 331] | – |
| T-stage at biopsy, N (%) | ||
| T1c | 5 (71.4) | 3 (100.0) |
| cT2a | 1 (14.3) | 0 |
| cT2b | 0 | 0 |
| cT2c | 1 (14.3) | 0 |
| T-stage at RP, N (%) | ||
| pT2a | 1 (14.3) | |
| pT2b | 1 (14.3) | 1 (33.3) |
| pT2c | 3 (42.9) | 1 (33.3) |
| pT3a | 1 (14.3) | 1 (33.3) |
| pT3b | 1 (14.3) | |
| ISUP-GGG at biopsy, N (%) | ||
| ISUP 1 | 0 | 1 (33.3) |
| ISUP 2 | 1 (14.3) | 1 (33.3) |
| ISUP 3 | 2 (28.6) | 0 |
| ISUP 4 | 2 (28.6) | 1 (33.3) |
| ISUP 5 | 2 (28.6) | 0 |
| ISUP-GGG at RP, N (%) | ||
| ISUP 2 | 3 (42.9) | 2 (66.6) |
| ISUP 3 | 3 (42.9) | 0 |
| ISUP 4 | 1 (14.3) | 1 (33.3) |
| ISUP 5 | 0 | 0 |
| Risk groups—National Comprehensive Cancer Network (NCCN) at biopsy, N (%) | ||
| Intermediate-risk | 3 (42.9) | 1 (33.3) |
| High-risk | 4 (57.1) | 2 (66.6) |
| Risk groups—National Comprehensive Cancer Network (NCCN) at RP, N (%) | ||
| Intermediate-risk | 5 (71.4) | 1 (33.3) |
| High-risk | 2 (28.6) | 2 (66.6) |
| Surgical margin status (R) | ||
| R0, N (%) | 4 (57.1) | 3 (100.0) |
| R1, N (%) | 3 (42.9) | 0 |
| R1 in pT2, N (% of all pT2) | 2 (40.0) | 0 |
| R1 in pT3, N (% of all pT3) | 1 (50.0) | 0 |
IQR, interquartile ranges; PSA, prostate specific antigen; SUV, standardized uptake value; PET, positron emission tomography; CLI, Cerenkov luminescence imaging; RP, radical prostatectomy; ISUP, International Society of Urological Pathology; GGG, Gleason grade groups; R0, negative surgical margin; R1, positive surgical margin.
After MRI-informed incision above the index lesion 2 out of the 3 prostates with PSM demonstrated elevated CLI signals in the index lesions (Figure 1). The anatomical correlation of the three patients with PSM is shown in Figure 2. Here the histopathological finding with a positive resection margin is shown with the corresponding CLI image. One patient had a TBR of 2.1 after application of the 550-nm OF with a decay corrected activity level of 1.3 kBq/mL at the time of CLI analysis with an Internal Society of Urologic Pathology (ISUP) grade group of 4 (Figure 1, A1, B1, C1; Figure 2, images 1.1). In addition, this patient showed another suspicious CLI signal on the dorsal right surface of the prostate. A further positive resection margin was histopathologically confirmed here (Figure 2, images 1.2). The second patient with PSM but negative CLI findings of the incised lesion showed a TBR of 1.5 after application of the 550-nm OF with a decay corrected activity level of 0.89 kBq/mL at the time of CLI analysis with an ISUP grade group of 2 (Figure 1, A2, B2, C2; Figure 2, images 2). The third patient with PSM and CLI signal showed a TBR of 2.15 after application of the 550-nm OF with a decay corrected activity level of 0.34 kBq/mL at the time of CLI analysis with an ISUP grade group of 2 (Figure 2, images 3).
Figure 1.
Grey-scale photographic images overlaid with Cerenkov signals, without the 550-nm optical short-pass filter (A) and with the 550-nm optical short-pass filter (B). The incised prostate above the index lesion is displayed in (C). The location of the incision is marked by a red dotted line on (A,B). The blue circles show the background ROIs. The suspicious CLI-intensity is labelled through green ROIs corresponding to the illuminating surface. (A1,B1,C1) Prostatectomy specimen with a positive resection margin as well as CLI-positivity of the incised lesion A high CLI-intensity at the left dorso-apical prostate is seen in both images without (TBR: 3.33, CNR: 16.2) and with (TBR: 2.1, CNR: 6.54) 550-nm optical short-pass filter and represented by an arrow. The incised specimen revealed a positive CLI signal corresponding with prostate cancer cells at this area (blue arrow). (A2,B2,C2) Prostatectomy specimen with a positive resection margin but CLI-negativity of the incised lesion A high CLI-intensity at the left apex of the prostate is seen in both images without (TBR: 2.79, CNR: 11.73) and with (TBR: 1.5, CNR: 2.44) 550-nm optical short-pass filter and represented by an arrow. The incised specimen revealed a negative CLI signal despite histopathological confirmation of prostate cancer cells at the incised area (blue arrow). (A3,B3,C3) One patient with negative resection margins and CLI-negativity of the incised lesion A high CLI-intensity at the bladder neck is seen (TBR: 3.36, CNR: 11.44), correlating with the usage of diathermy intraoperatively. After application of the 550-nm optical short-pass filter these signals are close the background intensity level (TBR: 1.1, CNR: 0.54). The incised specimen revealed a negative CLI signal despite histopathological confirmation of prostate cancer cells at the incised area (blue arrow). The star marks again an area of high CLI-intensity caused by diathermy since the specimen are imaged without the 550-nm optical short-pass filter. D, dorsal; R, right side; V, ventral; ROIs, regions of interest; CLI, Cerenkov luminescence imaging; TBR, tumor to background ratio; CNR, contrast to noise ratio.
Figure 2.
Correlation of the histopathological positive surgical margin (on the left) with the corresponding CLI image (on the right). For this purpose, the quarters have been combined into a whole-mount where applicable. In the histopathological image, the prostate carcinoma tissue is outlined in black and the R1 status is shown with a red line. In the CLI image, the corresponding signal is marked with an arrow. All CLI images were generated with the 550-nm optical filter. CLI, Cerenkov luminescence imaging; R, right; D, dorsal; V, ventral.
Activity levels at CLI varied according to different periods between tracer injection and CLI with a median of 0.38 kBq/mL (0.34; 1.3 kBq/mL). Importantly, all of the three men within the control group showed CLI signals, especially at the bladder neck and in areas with prior use of electrocautery (Figure 3). In all ten cases the addition of the 550-nm OF significantly reduced the area of displayed ROIs by 85% from a median of 1.8 to 0.15 cm2 (Table 2, P=0.005). Noteworthy, only false positive signals disappeared. Positive signals in CLI caused by PSM have not been affected by the application of the 550-nm OF. According to the histopathological results, three patients showed positive resection margins. All these patients had received prior tracer injection and all PSM were located at CLI suspicious areas and could be detected correctly with CLI. The median SI of the prostate background of the study group without 550-nm OF was 2,392.00 photons/s/cm2/sr (IQR, 1,849.17; 6,150.75 photons/s/cm2/sr). The median SI using the 550-nm OF was significantly reduced to 1,465.50 photons/s/cm2/sr (IQR, 1,373.00; 1,776.08 photons/s/cm2/sr) (P=0.018). The ratios, TBR and CNR, were not significantly changed by the optical filter (Table 3).
Figure 3.
Anatomical (A) and grey-scale photographic images overlaid with Cerenkov signals, without the 550-nm optical short-pass filter (B) and with the 550-nm optical short-pass filter (C). All images show patients of the control group. The blue circles are background ROIs. Areas of suspicious CLI-intensity are labelled with green ROIs. (A1,B1,C1) Prostatectomy specimen with a high CLI-intensity at the ventral bladder neck is seen and represented by an arrow (TBR: 7.32, CNR: 34.32), correlating with the usage of diathermy intraoperatively. After application of the 550-nm optical short-pass filter these signals are close the background intensity level. The location of the area is marked here with a dotted arrow (TBR: 1.10, CNR: 3.84). (A2,B2,C2) Prostatectomy specimen with a moderate CLI-intensity at the bladder neck is seen and represented by an arrow (TBR: 3.43, CNR: 15.4), also correlating with the usage of diathermy intraoperatively. After application of the 550-nm optical short-pass filter these signals are similar to that of the background. The location of the area is marked here with a dotted arrow (TBR: 0.99, CNR: −0.03). The star marks a foreign body (glove residue). (A3,B3,C3) Prostatectomy specimen with a moderate CLI-intensity at the ventro-apical side of the prostate is seen and represented by an arrow (TBR: 3.99, CNR: 16.7), also correlating with the usage of diathermy intraoperatively. After application of the 550-nm optical short-pass filter these signals are close the background intensity level. The location of the area is marked here with a dotted arrow (TBR: 1.38, CNR: 2.64). ROIs, regions of interest; CLI, Cerenkov luminescence imaging; TBR, tumor to background ratio; CNR, contrast to noise ratio.
Table 2. Cerenkov luminescence imaging measurements of each prostatectomy specimen.
| Patient number | Without 550-nm OF | With 550-nm OF | ||||||
|---|---|---|---|---|---|---|---|---|
| TBR | CNR | ROI area (cm2) | TBR | CNR | ROI area (cm2) | |||
| Study group | ||||||||
| 1 | 3.36 | 11.44 | 1.80 | 1.10 | 0.54 | 0 | ||
| 2 | 2.81 | 9.48 | 2.00 | 1.08 | 0.48 | 0.56 | ||
| 3 | 14.41 | 233.93 | 3.50 | 1.12 | 0.58 | 0.80 | ||
| 4 | 5.73* | 14.98* | 0.70* | 2.15* | 6.71* | 0.20* | ||
| 5 | 3.85 | 10.87 | 3.00 | 1.58 | 4.58 | 0.50 | ||
| 6 | 3.33* | 16.20* | 0.75* | 2.10* | 6.54* | 0.10* | ||
| 7 | 2.79* | 11.73* | 0.47* | 1.50* | 2.44* | 0.33* | ||
| Control group | ||||||||
| 8 | 3.99 | 16.70 | 1.80 | 1.38 | 2.64 | 0.08 | ||
| 9 | 3.43 | 15.40 | 0.60 | 0.99 | −0.03 | 0 | ||
| 10 | 7.32 | 34.32 | 2.70 | 1.10 | 3.84 | 0 | ||
*, specimens with positive surgical margins. Study group patient 1–7 and the control group patient 8–10. Measured intensities are stated as tumor to background ratio (TBR) and contrast to noise ratio (CNR). Measurements were either performed without a 550-nm optical short-pass filter or with a 550-nm optical short-pass filter. The area of regions of interests (ROIs) is stated in cm2. CNR, contrast to noise ratio; OF, optical short-pass filter.
Table 3. Cerenkov Luminescence measurements of the study group (n=7) grouped to positive surgical margins (n=3) and negative surgical margins (n=4).
| Data of the study group (n=7) | CLI Data without 550-nm short-pass filter | CLI Data with 550-nm short-pass filter | P value |
|---|---|---|---|
| Median prostate background, photons/s/cm2/sr (IQR) | 2,392.00 (1,849.17; 6,150.75) | 1,465.50 (1,373.00; 1,776.08) | 0.018 |
| Median TBR with PSM (IQR) | 3.33 (2.79; 5.73) | 2.10 (1.50; 2.15) | Not significant |
| Median TBR without PSM (IQR) | 3.61 (2.95; 11.77) | 1.11 (1.09; 1.47) | Not significant |
| Median CNR with PSM (IQR) | 14.98 (11.73; 16.20) | 6.54 (2.44; 6.71) | Not significant |
| Median CNR without PSM (IQR) | 11.16 (9.83; 178.31) | 0.56 (0.50; 3.58) | Not significant |
TBR and CNR are stated as median values (IQR). CLI, Cerenkov Luminescence Imaging; CNR, contrast to noise ratio; IQR, interquartile ranges; PSM, positive surgical margin; TBR, tumor to background ratio.
Four patients with negative resection margins showed no noticeable Cerenkov luminescence in the incised index lesion. Representative images are shown in Figure 1, A3, B3, C3. Decay corrected activity levels for the time of CLI investigation ranged from 0.33 to 0.78 kBq/mL. ISUP grade group of these patients ranged from 2 to 3.
Measurements of the CLI intensity with the 550-nm OF showed a significant Pearson’s correlation of 0.82 between PSM and the elevated TBR (P=0.003, Table 3). Measurements without the OF did not correlate significantly. Besides the TBR, the CNR was calculated, also demonstrating a significant Pearson’s correlation of 0.66 (P=0.04) (Tables 2,3).
The examination of the two groups—patients with preoperative 68-Ga-PSMA and without tracer injection—showed no significant differences between the examined TBR and CNR values if measured without OF highlighting the potential for false-positive CLI signals. After application of the OF a significant decrease of the TBR (P=0.02) was observed (Figure 3).
Discussion
This feasibility study evaluates the efficacy of intraoperative 68-Ga-PSMA CLI with an additional 550-nm OF for assessing tumor margin status in patients with primary PC. In general, CLI is able to detect PC cells close to the surface of the prostate gland as well as in lymph nodes (27,28,34). Using the additional filter did not affect the sensitivity for detection of PSM in PC. Positive findings by CLI were possible down to a minimal activity of 0.34 kBq/mL at the time of examination.
In all ten examined prostates increased CLI intensities could be detected without the 550-nm OF. As already described in a previous publication, these intensities are frequently found at the bladder neck. A moderate to strong PSMA-staining in benign tissues may cause these CLI signals (27). After investigating the same prostate alignment with an additional 550-nm OF, these signals were significantly reduced. To further investigate the origin of increased activity at non-cancerous surfaces, three patients undergoing RP without prior tracer injection were examined as a control group using CLI. After intraoperative prostate retrieval, the same procedure was performed as in patients with preoperative tracer injection. Interestingly, increased CLI intensities, mainly at the bladder neck, were also detected in these specimens, suggesting that this false positive, artificial signal is independent of tracer injection. Since the bladder neck, in contrast to the apex, is almost exclusively resected by electrocoagulation, it is reasonable to assume that the thermal alteration of the tissue generates radiation which is detected by the used system. Macroscopic examination of the suspect areas showed a strong correlation to areas of coagulated tissue. This was the case for both monopolar and bipolar devices. Accordingly, electrocoagulation was used sparsely in one patient, with a correspondingly lower occurrence of CLI signals. Importantly, here, we are able to demonstrate that these non-specific signals can be significantly reduced by using a 550-nm OF and therefore are assigned to a longer wavelength than the CLI signals of 68-Ga-PSMA-11.
68-Ga-PSMA-11 is known to be excreted via the urinary tract, and thus could also cause elevated signals at the bladder neck or prostate base through urinary contamination (35). One method for minimising the risk of prostate surface contamination from urine is to rinse the prostate with sodium chloride prior to CLI image acquisition. In the present study, the urine of all patients was drained through a urethral catheter immediately at the beginning of the surgery and all prostates were wiped to clean blood and fluids before the examination.
Noteworthy, the suspicious CLI signals at the bladder neck could be measured over 2 hours with approximately the same intensity. Therefore, the hypothesis that contamination by a PET tracer with a half-life of about 60 minutes leads to these signals is unlikely (29).
Intraoperative qualitative assessment of CLI data may lead surgeons to resect non-cancerous tissues, especially if no OF is used. Thus, quantitative assessment must be established to standardize CLI for multicentre and multi-surgeon validation trials. In two recently published studies, TBR measurements were used for quantitative assessment of CLI intensities (27,28). Grootendorst et al. demonstrated the clinical feasibility and safety of CLI in a small number of breast cancer patients using 18F-FDG with a mean TBR of 2.41 (range, 1.63–3.22) (25). In a prospective study of five primary PC patients undergoing RP Olde Heuvel et al. postulate a mean TBR >2 to sufficiently distinguish between cancerous and benign tissue (28). According to our findings, elevated TBR and CNR correlate significantly with PSM if CLI is measured using the 550-nm OF. In contrast neither elevated TBR nor CNR values without 550-nm OF, correlated with the presence of cancer cells close to the surface in our cohort. Reassuringly, our data are in line with the findings of Olde Heuvel et al. regarding the TBR. All patients with PSM and an increased CLI signal in the incision of the index lesion presented a TBR >2 (Table 3). The CNR values of this patient group were >6, indicating that a cut-off to distinguish between false-positive and PC-induced signal increases should be in this range.
In one patient with PSM, slightly elevated CLI intensities were seen without a positive CLI signal of the incised index lesion. The true-positive superficial signals were located in the area of the expected index lesion, left apex of the prostate (Figure 1, A2, B2, C2). Decay corrected 68-Ga-PSMA activity for the time of analysis was 0.89 kBq/mL, 316 minutes after tracer injection. In an experimental study the minimum detection limit was 1.2 kBq/mL for 68-Ga-PSMA. Here, Eppendorf tubes were filled with different amounts of tracer activities and examined for the minimal activity seen on CLI (36). Therefore, CLI might be more sensitive than previously thought. However, it remains unclear why we did not detect a signal in the incised specimen. Generally, underestimation of CLI signals could arise from the fact that some positrons escape from the surface of the specimen before their energy enter the Cerenkov threshold, which is likely for high energy Ga-68 positrons (37). According to Ciarrocchi et al. Cerenkov radiation is emitted on a cone around the particle direction. The characteristic emission angle depends on the velocity of the charged particle. The particle slows down depending on the emitted angle. Due to the lack of energy, however, no luminous phenomenon can be detected at a certain point. Therefore, it can be assumed that false positive findings are not influenced by the radiation angle of the charged particles. In addition, an evaluation of the specimens is performed in different positions (38). Another patient with a decay corrected activity of 0.34 kBq/mL, at the time of CLI analysis, demonstrated an elevated signal of the incised prostate gland with a TBR of 2.15. Contrary, no positive CLI signals of the incised index lesion were detectable in four patients with negative surgical margins and a decay-corrected activity at the time of CLI analysis below 1.2 kBq/mL. This is certainly mainly due to the low activity, but still contradicts the findings above where two PSM were detected at very low levels. This observation implies that there must be several other factors besides calculated activity, which have an influence on tracer accumulation and thus the CLI signal. It is known that low-grade cancers have a lower PSMA expression, resulting in a shorter period for positive CLI signals (39). In addition, other oncological features such as the size of the index lesion could also influence SI. Furthermore, heterogeneity of tracer activity in the prostate is a common finding which is in accordance with observations of different PSMA expression levels between patients, and even heterogeneity within the same prostatic lesion. Therefore, larger scale studies with standardized operating procedures are clearly needed to evaluate the potential and the field of application before widespread adoption of this promising technique. In the future, the immediate intraoperative evaluation of the entire prostate surface could reduce or replace a frozen section examination. This could save time and personnel resources.
Some limitations of our manuscript merit further discussion. First, this study addresses a small selective population since only patients with an elevated risk for PSM due to advanced cancer were selected. Therefore, these are not representative for all PC patients undergoing RP. Second, the administrated tracer activity varied between the patients as well as the time period from tracer injection to CLI. These challenges might be overcome in case of an intraoperative tracer injection to standardize these parameters. In our study setting, tracer injection was only allowed within the context of a PET/CT. This causes significant time delays until the entire prostate surface is examined for elevated CLI intensities. Last, in 4 patients with NSM, imaging of tumor tissue by CLI revealed no elevated signals. This may be due to several oncological characteristics as described above. In our opinion, however, this is mainly due to the short half-life of the tracer. Therefore, further studies with either an intraoperative tracer injection or a tracer with a longer half-life such as 18-F-PSMA are mandatory.
Conclusions
Intraoperative 68-Ga-PSMA CLI in PC is a tool that warrants further investigation to improve RP. CLI provides high-resolution information of the whole prostate gland that may allow surgeons to assess margin status with a good correlation to histopathologic examination. The intraoperative analysis of the entire prostate surface with CLI in combination with OF is a promising approach to reduce artificial signals. Nevertheless, further clinical studies are necessary to determine sensitivity and specificity.
Supplementary
The article’s supplementary files as
Acknowledgments
Funding: This work was supported by the “Deutsche Forschungsgemeinschaft - DFG” (Project number: Projekt HA 5160/5-1).
Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013) and after written informed consent of the patients. The study was approved by institutional ethics board of the University Duisburg-Essen (approval number 19-8749-BO).
Footnotes
Provenance and Peer Review: This article was commissioned by the Guest Editor (Tilman Todenhöfer) for the series “Management of Advanced Genitourinary Malignancies” published in Translational Andrology and Urology. The article has undergone external peer review.
Reporting Checklist: The authors have completed the STROBE reporting checklist. Available at http://dx.doi.org/10.21037/tau-20-1141
Data Sharing Statement: Available at http://dx.doi.org/10.21037/tau-20-1141
Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/tau-20-1141). The series “Management of Advanced Genitourinary Malignancies” was commissioned by the editorial office without any funding or sponsorship. Dr. CK reports personal fees from Bristol-Myer-Squibb, outside the submitted work; Dr. NNH reports personal fees from Boston Scientific, personal fees from Novartis, personal fees from Intuitive Surgical, personal fees from LightPoint Medical, outside the submitted work; Dr. HR reports personal fees from Roche, grants and personal fees from Bristol-Myers Squibb, personal fees from Philips, other from Bayer, outside the submitted work; Dr. WPF reports personal fees from RadioMedix, personal fees from Parexel, personal fees from Bayer, personal fees from BTG, personal fees from Endocyte, during the conduct of the study; Dr. KH reports personal fees from Bayer, personal fees and other from Sofie Biosciences, personal fees from SIRTEX, non-financial support from ABX, personal fees from Adacap, personal fees from Curium, personal fees from Endocyte, grants and personal fees from BTG, personal fees from IPSEN, personal fees from Siemens Healthineers, personal fees from GE Healthcare, personal fees from Amgen, personal fees from Novartis, personal fees from ymabs, outside the submitted work; Dr. Radtke reports personal fees from Invivo, personal fees from Uronav, personal fees from Bender group, personal fees from Becklemann and Partners, personal fees from Saegeling Medizintechnik, other from Advanced Accelerator Applications, other from Novartis, outside the submitted work; Dr. BAH reports personal fees from ABX, personal fees from Bayer, personal fees and non-financial support from Lightpoint medical, Inc., personal fees and non-financial support from Janssen R&D, grants from German Research Foundation, during the conduct of the study; personal fees and non-financial support from Bayer, personal fees and non-financial support from BMS, personal fees and non-financial support from AstraZeneca, personal fees from Pfizer, outside the submitted work; Dr. ST reports personal fees and other from Ipsen, personal fees from Eisai, personal fees from Bayer, personal fees from Janssen, personal fees from Novartis, personal fees from Bristol-Myers-Squibb, personal fees from BrachySolutions, outside the submitted work. The authors have no other conflicts of interest to declare.
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