FIG 1.

The arginine-rich-amphipathic helix domain (RAH) of ROP17 is sufficient to localize miniTurbo to the Toxoplasma PVM in infected cells. (A) Schematic of the miniTurbo fusion proteins expressed in HFFs depicting the epitope tag (V5), miniTurbo ligase (miniTurbo), nuclear export signal (NES), flexible linker (linker [amino acids KGSGSTSGSG]), and RAH domain of ROP17 (ROP17 RAH, [amino acids 104 to 223]). (B) Representative immunofluorescence microscopy images of tachyzoite-infected HFF populations stably expressing miniTurbo fusion proteins depicted in panel A. RHΔhptΔku80 tachyzoites were allowed to infect the corresponding HFFs for 24 h before the infected monolayers were fixed with methanol. The miniTurbo fusion proteins were detected with mouse anti-V5 antibodies (green), tachyzoites were detected with rabbit anti-SAG2A antibodies (magenta), and host and parasite nuclei were visualized using DAPI (blue). Scale bar is 10 μm.