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editorial
. 1999 Nov;37(11):3786–3787. doi: 10.1128/jcm.37.11.3786-3787.1999

Species versus Biotype Status

Donald E Woods 1,*
PMCID: PMC85768  PMID: 10610379

I was very disappointed to see that the pointless discussion regarding the species versus biotype status of Ara and Ara+ strains of Burkholderia pseudomallei continues (3). My laboratory has performed a number of studies on B. pseudomallei and B. pseudomallei-like strains, and we demonstrated using well-defined criteria that these should be classified as two separate species (1). In 1998, the International Journal of Systematic Bacteriology published a manuscript, based upon peer-reviewed studies from my laboratory, indicating tht B. pseudomallei-like strains should be awarded separate species status and that this species should be named Burkholderia thailandensis (2). The awarding of species status was based upon significant differences between B. thailandensis and B. pseudomallei, including distinctive nucleotide sequence differences in 16S rRNA (15 nucleotide dissimilarities), differences in lipopolysaccharide composition [B. thailandensis synthesizes a repeating disaccharide having the structure →3)-β-d-glucopyranose-(1→3)-6-deoxy-α-l-talopyranose-(1→; B. pseudomallei strains also produce this repeating disaccharide as well as a 1,3-linked homopolymer of 2-O-acetylated 6-deoxy-β-d-manno-heptopyranosyl residues (4, 5)], differences in colonial morphology on Ashdown's selective medium (B. thailandensis colonies are smooth and glossy with a pink pigmentation, while B. pseudomallei colonies are rough and wrinkled with a dark purple pigmentation [6]), biochemical differences (B. thailandensis assimilates l-arabinose, 5-keto-gluconate, and adonitol, and it does not utilize erythritol and dulcitol as carbon sources; B. pseudomallei does not assimilate l-arabinose, 5-keto-gluconate, and adonitol, and it does utilize erythritol and dulcitol as carbon sources [6]). B. thailandensis is not associated with human disease, and B. pseudomallei is associated with human disease (6), B. thailandensis is avirulent for Syrian golden hamsters (50% lethal dose [LD50] > 105), and B. pseudomallei is virulent for Syrian golden hamsters (LD50 < 100) (2).

In November 1998, at the International Congress on Melioidosis held in Bangkok, Thailand, the authors of the article recently published in the Journal of Clinical Microbiology (3) presented data published in abstract form, showing that distinctive nucleotide sequence differences in the 16S rRNA gene of Ara+ and Ara B. pseudomallei could be detected by a simple PCR system and that these nucleotide diffferences appeared to be species specific (7). Additionally, the same group proposed that the species name B. siamensis be given to this new species rather than B. thailandensis, since the former more appropriately describes the geographic location where the organism was first identified. The authors also indicated that Siam is the Thai name for Thailand, and the taxon siamensis has been used to describe many indigenous species found in Thailand. As the species name B. thailandensis clearly has precedent over B. siamensis, this proposal was not accepted.

When will the madness end?

REFERENCES

  • 1.Brett P J, DeShazer D, Woods D E. Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains. Epidemiol Infect. 1997;118:137–148. doi: 10.1017/s095026889600739x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Brett P J, DeShazer D, Woods D E. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol. 1998;48:317–320. doi: 10.1099/00207713-48-1-317. [DOI] [PubMed] [Google Scholar]
  • 3.Dharakul T, Tassaneetrithep B, Trakulsomboon S, Songsivilai S. Phylogenetic analysis of Ara+ and Ara−Burkholderia pseudomalleiisolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. J Clin Microbiol. 1999;37:1906–1912. doi: 10.1128/jcm.37.6.1906-1912.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Knirel Y A, Paramonov N A, Shaskov A S, Kochetkov N K, Yarullin R G, Farber S M, Efremko V I. Structure of the polysaccharide chains of Pseudomonas pseudomalleilipopolysaccharides. Carbohydr Res. 1992;233:185–193. doi: 10.1016/s0008-6215(00)90930-3. [DOI] [PubMed] [Google Scholar]
  • 5.Perry M B, MacLean L L, Scholaardt T, Bryan L E, Ho M. Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei. Infect Immun. 1995;63:3348–3352. doi: 10.1128/iai.63.9.3348-3352.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Suthiekanun B, Smith M D, Dance D A B, Walsh A L, Pitt T L, White N J. Biochemical characteristics of clinical and environmental isolates of Burkholderia pseudomallei. J Med Microbiol. 1996;45:408–412. doi: 10.1099/00222615-45-6-408. [DOI] [PubMed] [Google Scholar]
  • 7.Tassaneetrithep B, Songsivilai S, Kanistanon D, Trakulsomboon S, Dharakul T. Distinctive nucleotide sequence differences in the 16S rRNA gene of Ara+ and Ara− Burkholderia pseudomallei. 1998. p. 148. , abstr. P905. In Program and abstracts of the International Congress on Melioidosis. [Google Scholar]
J Clin Microbiol. 1999 Nov;37(11):3786–3787.

AUTHOR'S REPLY

Sirirurg Songsivilai 1,1-150

Our study describes the 16S rRNA gene sequences of Ara and Ara+ biotypes of B. pseudomallei (1-2). In fact from a total of 1,488 nucleotides there are 15 nucleotide differences, of which 11 are species or biotype specific (99.26% homology). Our interpretation is that the degree of differences is smaller than the differences among species in the genus Burkholderia. The data was discussed among those working in the melioidosis field. Therefore, based on this evidence we took a conservative approach and used “Ara+ B. pseudomallei” as was commonly used prior to the proposal of the new species named B. thailandensis in 1998 (1-1). In our article, however, the published reference on B. thailandensis has been adequately cited in the Discussion section (1-2). There is no reason not to use the name B. thailandensis if the data being presented support such a conclusion.

As for the status (species versus biotype) of Ara+ B. pseudomallei, Burkholderia-like organisms, and B. thailandensis, it is well recognized by the melioidosis community and me that such organisms differ from the classical B. pseudomallei in several important aspects, as described in the Discussion section (1-2). When such differences occur, a new species name is proposed (as in this case by Dr. Woods's group). It should be up to a group's scientific peers to obtain their own data on the organism, and if it passes the tests, the issuing of a new species and its name will be generally accepted. This topic was brought into a discussion at the International Congress in Melioidosis at the end of 1998 (1-3). In our article (1-2), we presented the result on one aspect of the topic while acknowledging the differences in several other aspects reported by others.

The name B. siamensis requires no discussion since it was not in the article published in 1998 (1-1) and was not used in our article (1-2) or elsewhere.

REFERENCES

  • 1-1.Brett P J, DeShazer D, Woods D E. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol. 1998;48:317–320. doi: 10.1099/00207713-48-1-317. [DOI] [PubMed] [Google Scholar]
  • 1-2.Dharakul T, Tassaneetrithep B, Trakulsomboon S, Songsivilai S. Phylogenetic analysis of Ara+ and Ara−Burkholderia pseudomalleiisolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. J Clin Microbiol. 1999;37:1906–1912. doi: 10.1128/jcm.37.6.1906-1912.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 1-3.Tassaneetrithep B, Songsivilai S, Kanistanon D, Trakulsomboon S, Dharakul T. Distinctive nucleotide sequence differences in the 16S rRNA gene of Ara+ and Ara− Burkholderia pseudomallei. 1998. p. 148. , abstr. P905. In Program and abstracts of the International Congress on Melioidosis. [Google Scholar]

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