Table 4.
Enzymes inhibitory capacity of extracts from Lavandula viridis L’Hér and Thymus lotocephalus López and Morales shoots cultured in media with 0 (control), 1, 5 or 10 mg/L of CuO, ZnO and Fe3O4 nanoparticles.
| Treatment | NP Concentration (mg/L) | TYR (mgKAE/gextract) | AChE (mgGE/gextract) | BChE (mgGE/gextract) |
|---|---|---|---|---|
| L. viridis | ||||
| Control | 0 | 13.27 ± 0.08 c | 4.43 ± 0.08 a | 9.74 ± 0.89 b |
| CuO | 1 | 14.90 ± 0.66 b,c | 3.44 ± 0.19 b | 10.71 ± 0.56 b |
| 5 | 17.49 ± 0.36 a | 3.65 ± 0.37 b | 10.26 ± 0.26 b | |
| 10 | 15.87 ± 0.36 a,b | 4.58 ± 0.26 a | 12.33 ± 0.31 a | |
| ZnO | 1 | 13.99 ± 0.47 c | 3.42 ± 0.15 b | 4.04 ± 0.21 d,e |
| 5 | 10.31 ± 0.34 d | 3.30 ± 0.21 b | 2.49 ± 0.51 f | |
| 10 | 13.35 ± 0.31 c | 3.34 ± 0.23 b | 2.96 ± 0.44 e,f | |
| Fe3O4 | 1 | 13.64 ± 0.41 c | 3.29 ± 0.19 b | 5.12 ± 0.52 c,d |
| 5 | 14.60 ± 0.66 b,c | 3.75 ± 0.19 b | 4.95 ± 0.23 c,d | |
| 10 | 13.39 ± 0.08 c | 3.63 ± 0.26 b | 6.13 ± 0.65 c | |
| T. lotocephalus | ||||
| Control | 0 | 13.18 ± 0.58 d,e | 3.28 ± 0.19 a,b,c | 5.00 ± 0.20 d |
| CuO | 1 | 11.46 ± 0.67 e | 3.25 ± 0.28 a,b,c | 5.32 ± 0.32 c,d |
| 5 | 14.51 ± 0.97 b,c,d | 3.44 ± 0.12 a,b | 8.75 ± 0.60 a | |
| 10 | 14.16 ± 0.31 c,d,e | 3.05 ± 0.12 b,c | 6.36 ± 0.28 c | |
| ZnO | 1 | 13.88 ± 0.60 c,d,e | 2.86 ± 0.21 c | 3.38 ± 0.30 e |
| 5 | 15.49 ± 0.70 a,b,c,d | 3.05 ± 0.11 b,c | 4.92 ± 0.29 d | |
| 10 | 18.36 ± 1.51 a | 2.90 ± 0.18 b,c | 6.51 ± 0.67 b,c | |
| Fe3O4 | 1 | 17.39 ± 0.74 a,b | 3.74 ± 0.13 a | 4.92 ± 0.61 d |
| 5 | 15.68 ± 1.36 a,b,c,d | 2.90 ± 0.11 b,c | 5.27 ± 0.09 c,d | |
| 10 | 16.81 ± 1.04 a,b,c | 3.22 ± 0.12 a,b,c | 7.70 ± 0.32 b |
Values are expressed as mean ± standard error. For each variable and plant species values followed by different letters (a to e) are significantly different at p < 0.05 (Duncan’s New Multiple Range Test). TYR: tyrosinase; AChE: acetylcholinesterase; BChE: butyrylcholinesterase; KAE: kojic acid equivalents; GE: galantamine equivalents.