Figure 2.

The expression of miR-506 and CDK6 in lung cancer cells

(A) Detection of miR-506 and CDK6 in the lung cancer patients. The expression levels of miR-506 and CDK6 were tested in lung cancer patients (n = 77). The expression level of each gene in lung cancer tissue was normalized to paired normal tissue. The normalized expression levels of CDK6 and miR-506 were plotted. U6 was used as a reference gene for miR-506. The mRNA expression was normalized to GAPDH.

(B) Detection of miR-506 and CDK6 in the normal lung cell line and lung cancer cell lines. Western blot (left) detection of CDK6 in normal lung cell line MRC5, HLF, and lung cancer cell lines, including A549, 95D, H1975, H460, HCC827, H661, PC9, H23, H358, and H1299. Cells were grown into confluence. Then Cells were harvested for RT-qPCR and western blot analysis. β-Actin was used as a reference gene for western blot. U6 was used as a reference gene for miR-506. The mRNA expression was normalized to β-Actin.

(C) CDK6 protein levels in different lung cancer cells. Lung cancer cells were transfected with no target control (NC) and miR-506. Forty-eight hours after transfection, cells were collected for western blot. β-Actin was used as a reference gene for western blot.

(D) The CCK-8 cell viability assay in lung cancer cell lines. Forty-eight hours after transfection, cell viability was tested with CCK-8 assay. Data are presented as the mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, two-tailed Student’s t test.