Agarose gel electrophoresis of the amplified acc6’-Ib gene (4A), aadA gene (4B), and aph3’-VI gene (4C) from the ESBL-producing P. aeruginosa isolates.
(4A): Lane M: 100bp DNA Ladder; Lane C+: Positive Control; Lane C-: Negative Control; Lanes 1, 2, 4–6 are isolates that were positive for the acc6’-Ib gene (=395bp); Lane 3 is an isolate that was negative for the acc6’-Ib gene.
(4B): Lane M: 100bp DNA Ladder; Lane C+: Positive Control; Lane C-: Negative Control; Lanes 1–6 are isolates that were positive for the aadA gene (= 812bp).
(4C): Lane M: 100bp DNA Ladder; Lane C+: Positive Control; Lane C-: Negative Control; Lanes 3, 5, 10 are isolates that were positive for the aph3’-VI gene(=716bp); Lanes 1, 2, 4, 6–8 are isolates that were negative for the aph3’-VI gene.