Table 2:

Primers used for the detection of oprD, ESBL-related, MBL-related genes, and ERIC sequences in the PA isolates.

Target OprD	Primer	Sequence 5′ to 3′	AT °C	Size (bp)	Reference
oprD	oprD-F	GGAACCTCAACTATCGCCAAG	57	1412	30
	oprD-R	GTTGCCTGTCGGTCGATTAC
Target ESBLs	Primer	Sequence 5′ to 3′	AT °C	Size (bp)	Reference
blaTEM	TEM-F	ATGAGTATTCAACATTTCCGTG	55	840	31
	TEM-R	TTACCAATGCTTAATCAGTGAG
blaSHV	SHV-F	TTTATGGCGTTACCTTTGACC	53	1051	32
	SHV-R	ATTTGTCGCTTCTTTACTCGC
blaGES	GES1-F	ATGCGCTTCATTCACGCAC	55	860	33
	GES1-R	CTATTTGTCCGTGCTCAGG
blaPER	PER-F	GTAGTATCAGCCCAATCCCC	55	738	34
	PER-R	CCAATAAAGGCCGTCCATCA
blaVEB	VEB-F	GGAACAACTTTGACGATTGA	57	374	34
	VEB-R	CCCTGTTTTATGAGCAACAA
bla CTXM2	CTX-M- 2-F	ATGATGACTCAGAGCATTCGC	53	749	35
	CTX-M- 2-R	GATATCGTTGGTGGTGCCA
Target MBLs	Primer	Sequence 5′ to 3′	AT °C	Size (bp)	Reference
blaNDM- 1-like	NDM-1- F	GCGAACACACAGCCTGACTTT	57	813	33
	NDM-1- R	CAGCCACCAAAAGCGATGTC
blaIMP	IMP-F	CATACTCGTTGAAGAAGTTAAC GG	53	448	35
	IMP-R	GAGAATTAAGCCACTCTATTGC
blaVIM	VIM-F	TGGTCTACATGACCGCGTCT	53	766	3
	VIM-R	CGACTGAGCGATTTGTGTG
Target AME	Primer	Sequence 5′ to 3′	AT °C	Size (bp)	Reference
aac6’-Ib	aac(6’)Ib-F	TATGAGTGGCTAAATCGAT	49	395	37
	aac(6’)Ib-R	CCCGCTTTCTCGTAGCA
aadA	aadA-F	TTGTACGGCTCCGCAGTG	53	812	38
	aadA-R	CCCAATTTGTGTAGGGCTTA
aph3’-VI	aph(3’)VI-F	CGGAAACAGCGTTTTAGA	39	716	37
	aph(3’)VI-R	TTCCTTTTGTCAGGTC
Target ERIC	Primer	Sequence 5′ to 3′	AT	Size (bp)	Reference
ERIC2	ERIC2	AAGTAAGTGACTGGGGTGACGC	30	-	39

bp: base-pairs