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. 2020 Feb 13;4(1):2. doi: 10.3390/epigenomes4010002

Figure 3.

Figure 3

Monocyte-specific knockout of QKI ameliorates interstitial fibrosis upon unilateral urethral obstruction (UUO). (A) Schematic representing the genomic architecture of the conditional QKI knockout mouse (not to scale). (B) Relative expression level of the mRNA levels of the floxed exon 2 of the QKI mRNA in 10-day CLK and UUO kidneys normalized to the mRNA of β-actin is shown. (C) Sirius red staining for collagen in 4 μm sections of 10-day UUO kidneys derived from qkIFL/FL;LysM-cre or wild-type littermate controls. (D) Colorimetric quantitation of Sirius Red staining using ImageJ software. (E) mRNA levels of macrophage markers (CD115, F4/80) in whole kidney lysates derived from either qkIFL/FL;LysM-cre mice (gray bars) or wild-type littermate controls (open bars) on day 10 after UUO. (F) mRNA levels of fibrosis markers (α-SMA, COL1A1) in whole kidney lysates derived from either qkIFL/FL;LysM-cre mice (gray bars) or wild-type littermate controls (open bars) on day 10 after UUO. * p ≤ 0.05 by Students’ t-test, error bars represent SEM.