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. 2021 Nov 16;10:e67396. doi: 10.7554/eLife.67396

Figure 1. Single-cell transcriptomics reconstructs leukocyte subsets infiltrating the eye.

(A) Uniform manifold approximation and projection (UMAP) projection of seven pooled samples (control n = 1; B27-AU n = 2; B27+ AAU n = 4). The single-cell (sc) transcriptomes were manually annotated to cell types based on marker gene expression and distinguished in 13 cell clusters (color-coded; each dot represents one cell). (B) The mean proportion of cells (%) in each cluster per group is depicted in a stacked bar plot. (C) Dot plot of selected marker genes grouped by cluster. The average gene expression level is color-coded, and the circle size represents the percentage of cells expressing the gene. Threshold was set to a minimum of 10% of cells in the cluster expressing the gene. DC: dendritic cell, pDC: plasmacytoid DC, matDC: mature DC; granulo: granulocytes, NK cells: natural killer cells, gdTC: γδ T cells, Treg cells: regulatory T cells, Bc: B cells.

Figure 1.

Figure 1—figure supplement 1. Feature plots of lineage marker genes.

Figure 1—figure supplement 1.

Expression of lineage markers ITGAX, AXL, CLEC10A, MRC1, CLEC4C, IL3RA, CD83, LAMP3, CCL2, S100A8, CD33, S100A12, CD4, CD3G, CD8A, TRBC2, FOXP3, IL2RA, GNLY, IL7R, TRDC, NKG7, MS4A1, and IGHG1 is shown as feature plots. Each dot represents one cell.