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Accuracy of electrode localization assessed for the SPIM workflow. A, The peak locations of evoked activity and eYFP fluorescence were estimated using a Gaussian fit (4 mice, 17 penetrations). B, The distance between the peak locations of evoked activity and eYFP fluorescence. Absolute value of the difference in peak locations was used to quantify electrode localization accuracy (0.069 ± 0.054 mm, mean ± SD).
