| Strain, strain background (Mus musculus, male) |
BALB/cAJcl-nu/nu |
CLEA |
|
Ten-week-old |
| Cell line (Homo sapiens) |
Lenti-X 293T |
Takara Bio Inc |
|
|
| Cell line (Homo sapiens) |
HeLaS3 |
K.Matsumoto (Nagoya University, Aichi, Japan) |
|
RRID:CVCL_0058
|
| Cell line (Homo sapiens) |
S2-CP8 |
Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University |
|
RRID:CVCL_F971
|
| Cell line (Homo sapiens) |
PANC-1 |
RIKEN Bioresource Center Cell Bank |
|
RRID:CVCL_0480
|
| Cell line (Homo sapiens) |
BxPC-3 |
American Type Culture Collection |
|
RRID:CVCL_0186
|
| Cell line (Homo sapiens) |
HPAF-II |
American Type Culture Collection |
|
RRID:CVCL_0313
|
| Transfected construct (Homo sapiens) |
ARL4C-EGFP |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CG2A-EGFP |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CT27N-EGFP |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CQ72L-EGFP |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CΔPBR-EGFP |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CG2A-EGFP-Cterm |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CΔPBR-EGFP-Cterm |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CG2A-EGFP-GRP1PH |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4CG2A-EGFP-PLCδPH |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4C-mCherry |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4C-tdTomato |
This paper |
|
Various mutants of ARL4C |
| Transfected construct (Homo sapiens) |
ARL4C-FLAG-HA |
This paper |
|
Various mutants of ARL4C |
| Biological sample (Homo sapiens) |
Resected specimens of 57 patients with PDAC |
Osaka University |
|
|
| Antibody |
ARL4C (rabbit polyclonal) |
Atlas Antibodies |
#HPA028927 |
(WB 1:1000, IHC 1:50) |
| Antibody |
Clathrin (mouse monoclonal) |
BD Biosciences |
#610,500 |
(WB 1:1000) |
| Antibody |
EGR1 (rabbit monoclonal) |
Cell Signaling Technology |
#4,153 S |
(WB 1:1000) |
| Antibody |
β-catenin (mouse monoclonal) |
BD Biosciences |
#610,154 |
(WB 1:1000) |
| Antibody |
Ras (G12D) (rabbit monoclonal) |
Cell Signaling Technology |
#14,429 S |
(WB 1:1000) |
| Antibody |
Hsp90 (mouse monoclonal) |
BD Biosciences |
#610,419 |
(WB 1:1000) |
| Antibody |
HA (mouse monoclonal) |
BioLegend |
#901,502 |
(WB 1:1000) |
| Antibody |
HA (rat monoclonal) |
Roche |
#1867423001 |
(ICC 1:100) |
| Antibody |
GFP (rabbit polyclonal) |
Life Technologies/Thermo Fisher Scientific |
#A6455 |
(WB 1:4000) |
| Antibody |
GFP (mouse monoclonal) |
Santa Cruz Santa Cruz Biotechnology |
#sc-9996 |
(WB 1:1000) |
| Antibody |
FLAG (mouse monoclonal) |
WAKO |
#014–22,383 |
(WB 1:1000) |
| Antibody |
IQGAP1 (mouse monoclonal) |
Santa Cruz Santa Cruz Biotechnology |
#sc-376021 |
(WB 1:1000, IHC 1:800, ICC 1:100) |
| Antibody |
MMP14 (rabbit monoclonal) |
Abcam |
#ab51074 |
(WB 1:1000, IHC 1:200, ICC 1:100) |
| Antibody |
Cytohesin2 (rabbit polyclonal) |
Proteintech Group, Inc |
#67185–1-Ig |
(ICC 1:100) |
| Antibody |
Rac1 (mouse monoclonal) |
BD Biosciences |
#610,651 |
(WB 1:1000) |
| Antibody |
Cdc42 (rabbit polyclonal) |
Cell Signaling Technology |
#2,466 S |
(WB 1:1000) |
| Antibody |
CK19 (rabbit monoclonal) |
Abcam |
#ab52625 |
(IHC 1:100) |
| Antibody |
Mitochondria (mouse monoclonal) |
Merck Millipore |
#MAB1273 |
(IHC 1:100) |
| Antibody |
LYVE-1 (rabbit polyclonal) |
Abcam |
#ab14917 |
(IHC 1:100) |
| Antibody |
YAP/TAZ (rabbit monoclonal) |
Cell Signaling Technology |
#8,418 S |
(ICC 1:100) |
| Antibody |
Paxillin (mouse monoclonal) |
BD Biosciences |
#610,052 |
(ICC 1:100) |
| Antibody |
FAK(mouse monoclonal) |
BD Biosciences |
#610,087 |
(ICC 1:100) |
| Antibody |
P-Paxillin (Y118)(rabbit polyclonal) |
Cell Signaling Technology |
#2,541 S |
(ICC 1:100) |
| Antibody |
P-FAK (Y397)(rabbit monoclonal) |
Life Technologies/Thermo Fisher Scientific |
#44,625 G |
(ICC 1:100) |
| Antibody |
Cortactin(mouse monoclonal) |
Merck Millipore |
#05–180 |
(ICC 1:100) |
| Antibody |
ARL4C (rabbit polyclonal) |
This paper |
SAJ5550275 |
(ICC 1:100) |
| Recombinant DNA reagent |
pEGFPC2-IQGAP1 (plasmid) |
K.Kaibuchi (Nagoya University, Japan) |
|
|
| Recombinant DNA reagent |
pEGFP-mCyth2 (plasmid) |
J.Yamauchi (Tokyo University of Pharmacy and Life Science, Japan), |
|
|
| Recombinant DNA reagent |
pAcGFP-mPlcd1PH (plasmid) |
M.Matsuda (Kyoto University, Kyoto, Japan) |
|
|
| Recombinant DNA reagent |
CSII-CMV-MCS-IRES2-Bsd (plasmid) |
H.Miyoshi (RIKEN Bioresource Center, Ibaraki, Japan) |
|
|
| Recombinant DNA reagent |
pEGFPN3-hARL4C (plasmid) |
A.Kikuchi (Osaka University, Osaka, Japan) |
|
|
| Recombinant DNA reagent |
pEGFPN3-hGRP1 (plasmid) |
This paper |
|
Full length cDNAs of GRP1 ORF were reversely transcribed from mRNA extracted from MCF-7 cells. |
| Recombinant DNA reagent |
pEGFPN3-hMMP14 (plasmid) |
This paper |
|
Full length cDNAs of MMP14 ORF were reversely transcribed from mRNA extracted from U2OS cells. |
| Recombinant DNA reagent |
mRFP-FKBP-5-ptase-dom |
Addgene |
67,516 |
|
| Recombinant DNA reagent |
PM-FRB-CFP |
Addgene |
67,517 |
|
| Sequence-based reagent |
siRNA: randomized control |
This paper |
|
5'-CAGTCGCGTTTGCGACTGG-3' |
| Sequence-based reagent |
siRNA: human IQGAP1#1
|
This paper |
|
5'-GCTGCACATAGTTGCCTTT-3' |
| Sequence-based reagent |
siRNA: human IQGAP1#2
|
This paper |
|
5'-CCCTAATGTAGAATGTCAT-3' |
| Sequence-based reagent |
siRNA: human CYTH2#1
|
This paper |
|
5'-GGATGGAGCTGGAGAACAT-3' |
| Sequence-based reagent |
siRNA: human CYTH2#2
|
This paper |
|
5'-GCAGTTTCTATGGAGCTTT-3' |
| Sequence-based reagent |
siRNA: human ARPC2#1
|
This paper |
|
5'-GCCTATATTCACACACGTA-3' |
| Sequence-based reagent |
siRNA: human ARPC2#2
|
This paper |
|
5'-CCTATATTCACACACGTAT-3' |
| Sequence-based reagent |
siRNA: human MMP14#1
|
This paper |
|
5'-GCAGCCTCTCACTACTCTT-3' |
| Sequence-based reagent |
siRNA: human MMP14#2
|
This paper |
|
5'-CCGACATCATGATCTTCTT-3' |
| Sequence-based reagent |
siRNA: human KRAS#1
|
This paper |
|
5'-GCATCATGTCCTATAGTTT-3' |
| Sequence-based reagent |
siRNA: human KRAS#2
|
This paper |
|
5'-GTTGGAGCTGATGGCGTAG-3' |
| Sequence-based reagent |
siRNA: human CTNNB1#1
|
This paper |
|
5'-CCCACTAATGTCCAGCGTT-3' |
| Sequence-based reagent |
siRNA: human CTNNB1#2
|
This paper |
|
5'-GCATAACCTTTCCCATCAT-3' |
| Sequence-based reagent |
Antisense oligonucleotide: randomized control |
This paper |
|
T(Y)^a^g^A(Y)^g^a^G(Y)^t^a^5(Y)^c^c^A(Y)^t^c (Lower case = DNA; N(Y) = AmNA; 5(Y) = AmNA_mC; ^ = Phosphorothioated) |
| Sequence-based reagent |
Antisense oligonucleotide: ARL4C-1316 |
This paper |
|
G(Y)^5(Y)^A(Y)^t^a^c^c^t^c^a^g^g^T(Y)^A(Y)^a (Lower case = DNA; N(Y) = AmNA; 5(Y) = AmNA_mC; ^ = Phosphorothioated) |
| Sequence-based reagent |
human GAPDH_F |
This paper |
PCR primers |
5'-TCCTGCACCACCAACTGCTT-3' |
| Sequence-based reagent |
human GAPDH_R |
This paper |
PCR primers |
5'-TGGCAGTGATGGCATGGAC-3' |
| Sequence-based reagent |
human B2M_F |
This paper |
PCR primers |
5'-TGCTGTCTCCATGTTTGATGTATC-3 |
| Sequence-based reagent |
human B2M_R |
This paper |
PCR primers |
5'-TCTCTGCTCCCCACCTCTAAG-3' |
| Sequence-based reagent |
human ARL4C_F |
This paper |
PCR primers |
5'-AGGGGCTGTGAAGCTGAGTA-3’ |
| Sequence-based reagent |
human ARL4C_R |
This paper |
PCR primers |
5'-TTCCAGGCTGAAAAGCAGTT –3' |
| Sequence-based reagent |
human ARPC2_F |
This paper |
PCR primers |
5'-AGATTTCGATGGGGTCCTCT-3' |
| Sequence-based reagent |
human ARPC2_R |
This paper |
PCR primers |
5'-CCGGAAGATTTTCAAGGTCA-3' |
| peptide, recombinant protein |
FLAG peptide |
Sigma-Aldrich |
F3290 |
|
| Commercial assay or kit |
Lipofectamine2000 transfection reagent |
Life Technologies/Thermo Fisher Scientific |
11668019 |
|
| Commercial assay or kit |
Lipofectamine LTX reagent |
Life Technologies/Thermo Fisher Scientific |
15338100 |
|
| Commercial assay or kit |
RNAiMAX |
Life Technologies/Thermo Fisher Scientific |
13778075 |
|
| Commercial assay or kit |
ViaFect |
Promega Corp. |
E4981 |
|
| Commercial assay or kit |
TrypLE Express Enzyme |
Thermo Fisher Scientific |
12604013 |
|
| Commercial assay or kit |
PrimeSTAR Max DNA Polymerase |
Takara Bio Inc |
R045A |
|
| Commercial assay or kit |
In-Fusion HD Cloning Kit |
Clontech |
639,649 |
|
| Commercial assay or kit |
DakoReal EnVision Detection System |
Dako |
K500711-2 |
|
| Commercial assay or kit |
Peroxidase-Blocking Solution |
Dako |
S202386-2 |
|
| Commercial assay or kit |
G-Block |
GenoStaff |
GB-01 |
|
| Commercial assay or kit |
Blocking One Histo |
nacalai tesque |
06349–64 |
|
| Commercial assay or kit |
rat tail type I collagen |
Corning Inc |
354,236 |
|
| Commercial assay or kit |
DQ-collagen type I |
Invitrogen |
D12060 |
|
| Commercial assay or kit |
3D microfluidic cell culture chip |
AIM Biotech |
DAX-1 |
|
| Commercial assay or kit |
QCM Gelatin Invadopodia Assay (Red) |
Merck Millipore |
ECM671 |
|
| Commercial assay or kit |
poly-D-lysine |
Sigma-Aldrich |
P6407 |
|
| Commercial assay or kit |
Matrigel Growth Factor Reduced |
Corning Inc |
354,230 |
|
| Commercial assay or kit |
6.5 mm Transwell with 8.0 µm Pore Polycarbonate Membrane Insert |
Corning Inc |
3,422 |
|
| Commercial assay or kit |
BioCoat Matrigel Invasion Chambers with 8.0 µm PET Membrane |
Corning Inc |
354,480 |
|
| Commercial assay or kit |
Annexin V-FITC Apoptosis Detection Kit |
nacalai tesque |
15342–54 |
|
| Commercial assay or kit |
protein A Sepharose beads |
GE Healthcare |
17078001 |
|
| Commercial assay or kit |
Dynabeads Protein G |
Thermo Fisher Scientific |
DB10003 |
|
| Commercial assay or kit |
Pierce Silver Stain for Mass Spectrometry |
Thermo Fisher Scientific |
24,600 |
|
| Commercial assay or kit |
O.C.T. Compound |
Sakura Finetek |
|
|
| Commercial assay or kit |
NucleoSpin RNA |
MACHEREY-NAGEL GmbH & Co. KG |
740,955 |
|
| Commercial assay or kit |
ReverTra Ace qPCR RT Master Mix |
TOYOBO |
FSQ-201 |
|
| Commercial assay or kit |
Pierce BCA Protein Assay Kit |
Thermo Fisher Scientific |
23,227 |
|
| Chemical compound, drug |
PD184161 |
Sigma-Aldrich |
PZ0112 |
|
| Chemical compound, drug |
U0126 |
Promega Corp. |
V1121 |
|
| Chemical compound, drug |
Rapamycin |
Cell Signaling Technology |
9,904 |
|
| Chemical compound, drug |
LY294002 |
Cell Signaling Technology |
9,901 |
|
| Chemical compound, drug |
VivoGlo luciferin |
Promega Corp. |
P1043 |
|
| Software, algorithm |
HALO |
Indica Labs |
|
RRID:SCR_018350
|
| Software, algorithm |
NanoZoomer-SQ |
Hamamatsu Photonics K.K. |
|
|
| Software, algorithm |
UCSC Xena browser |
http://xena.ucsc.edu
|
|
RRID:SCR_018938
|
| Software, algorithm |
Kaplan–Meier plotter |
http://www.kmplot.com
|
|
RID:SCR_018753 |
| Software, algorithm |
GraphPad Prism 8 |
GraphPad Software. |
|
RRID:SCR_002798
|
| Software, algorithm |
Excel Toukei |
ESUMI Co., Ltd. |
|
|
| Software, algorithm |
Imaris |
Bitplane |
|
RRID:SCR_007370
|
| Software, algorithm |
Image J |
National Institutes of Health |
|
RRID:SCR_003070
|
| Software, algorithm |
Living Image 4.3.1 Software |
Caliper Life Sciences |
|
RRID:SCR_014247
|
| Software, algorithm |
ikra v1.2.2 |
https://zenodo.org/record/3606888 (Yu et al., 2019) |
|
|
| Software, algorithm |
iDEP.90 |
http://bioinformatics.sdstate.edu/idep90/ (Ge et al., 2020) |
|
|
| Software, algorithm |
Ingenuity Pathway Analysis |
IPA; Qiagen |
|
RRID:SCR_008653
|
| Other |
LSM880 laser scanning microscope |
Carl Zeiss |
|
|
| Other |
BZ-9000 |
Keyence |
|
|
| Other |
IVIS imaging system |
Xenogen Corp. |
|
|
| Other |
Hoechst33342 |
Invitrogen |
H1399 |
|
| Other |
Alexa Fluor 488 Phalloidin |
Invitrogen |
A12379 |
|
| Other |
Alexa Fluor 546 Phalloidin |
Invitrogen |
A22283 |
|
| Other |
Alexa Fluor 647 Phalloidin |
Invitrogen |
A22287 |
|
