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. 2021 Oct 29;148(21):dev199644. doi: 10.1242/dev.199644

Fig. 2.

Fig. 2.

Fam71f1 and Fam71f2 are required for normal male fertility and sperm head formation. (A) Fertility of Fam71f1-mutant males. Fam71f1+/−4 or Fam71f1−4/−4 males were mated with wild-type (WT) females and the number of pups born per plug was counted. Although 25 plugs were observed, Fam71f1−4/−4 males did not sire any offspring. (B) Fertility of Fam71f2-mutant males. Fam71f2+/+ or Fam71f2−9516/−9516 males were mated with WT females and the number of pups born per plug was counted. The fertility of Fam71f2−9516/−9516 males was significantly decreased compared with WT. (C) Observation of spermatozoa. Spermatozoa from Fam71f1- and Fam71f2-mutant mice lacked hook-like structures at the apical regions of sperm heads (arrowheads). Higher magnification images are shown in the top-right corners. (D) Immunofluorescence staining of the acrosome and mitochondria. Both Fam71f1- and Fam71f2-mutant spermatozoa showed abnormal morphology of the acrosome, whereas the mitochondrial sheaths were normal. Nuclei are stained blue (Hoechst), acrosomes green (lectin-PNA) and mitochondria magenta (TOM20). Data are mean±s.d.; ***P<0.001 (unpaired Student's t-test). Scale bars: 20 µm in C; 5 µm in D.