KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal anti-CD45, APC-eFlour780 | ThermoFisher | Cat #:47-0451-80; clone: 30-F11; RRID: AB_1548790; dilution: 1 to 40 |
| Rat anti mouse/human CD44, Brilliant Violet 785 | Biolegend | Cat #: 103059; clone: IM7; RRID: AB_2571953; dilution: 1 to 400 |
| Rat anti mouse/human CD44, Alexa Flour 700 | Biolegend | Cat #: 103026; clone: IM7; RRID: AB_493713; dilution 1 to 200 |
| Mouse anti human Ki-67, Alexa Flour 700 | BD Biosciences | Cat #: 561277; clone: B56; RRID: AB_10611571; dilution: 1 to 200 |
| Mouse anti Ki-67, BV786 | BD Biosciences | Cat #: 563756; clone: B56; RRID: AB_2732007; dilution: 1 to 200 |
| Rat anti mouse CD8a, BUV395 | BD Biosciences | Cat# 563786; Clone: 53-6.7; RRID: AB_2732919; dilution: 1 to 400 |
| Rat anti mouse CD8a, BUV737 | BD Biosciences | Cat #: 612759; Clone: 53-6.7; RRID: AB_2722580; dilution 1 to 400 |
| Rat anti mouse CD8a, APC | Biolegend | Cat #: 100712; Clone: 53-6.7; RRID: AB_312751; dilution 1 to 200 |
| Rat anti mouse CD366 (Tim-3), Brilliant Violet 605 | Biolegend | Cat #: 119721; Clone: RMT3-23; RRID: AB_2616907; dilution: 1 to 200 |
| Rat anti mouse CD366 (Tim-3), Brilliant Violet 785 | Biolegend | Cat #: 119725; Clone: RMT3-23; RRID: AB_2716066; dilution 1 to 100 |
| Rat monoclonal anti TIGIT, FITC | Thermo Fisher | Cat #: 11-9501-82; Clone: GIGD7; RRID: AB_2637373; dilution: 1 to 200 |
| Rat anti mouse CD279 (PD-1), Brilliant Violet 510 | Biolegend | Cat #: 135241; Clone: 29F.1A12; RRID: AB_2715761; dilution: 1 to 400 |
| Rat anti mouse CD223 (Lag-3), BUV737 | BD Biosciences | Cat #: 741820; Clone: C9B7W; dilution: 1 to 200 |
| Rat anti mouse CD244.2 (2B4), PE-Cyanine7 | Thermo Fisher | Cat #: 25-2441-82; Clone: eBio244F4; RRID: AB_2573432; dilution: 1 to 200 |
| Rat anti mouse TNF-alpha, PE | Biolegend | Cat #: 506306; Clone: MP6-XT22; RRID: AB_315427; dilution: 1 to 200 |
| Rat anti mouse IFN-gamma, FITC | Biolegend | Cat #: 505806; Clone: XMG1.2; RRID: AB_315400; dilution 1 to 100 |
| Rat anti mouse IFN-gamma, PerCP/Cyanine5.5 | Biolegend | Cat #: 505822; Clone: XMG1.2; RRID: AB_961359; dilution: 1 to 100 |
| Rat anti mouse CD8b (Ly-3), Alexa Flour 647 | Biolegend | Cat #: 126612; Clone: YTS156.7.7; RRID: AB_2075777; dilution 1 to 200 |
| Rat anti mouse CD326 (Ep-CAM) | Biolegend | Cat #: 118208; Clone: G8.8; RRID: AB_1134107; dilution 1 to 200 |
| Streptavidin, R-Phycoerythrin Conjugate (SAPE) | Invitrogen | Cat #: S21388 |
| Rabbit monoclonal anti TCF1/TCF7, Alexa Flour 647 | Cell Signaling Technology | Cat #: 6709S; Clone: C63D9; RRID: AB_2797631; dilution: 1 to 250 |
| Rabbit monoclonal anti TCF1/TCF7, Alexa Flour 488 | Cell Signaling Technology | Cat #: 6444S; Clone: C63D9; RRID: AB_2797627; dilution 1 to 400 |
| Armenian hamster anti mouse CD183 (CXCR3), BV421 | BD Biosciences | Cat #: 562937; Clone: CXCR3-173; RRID: AB_2687551; dilution: 1 to 200 |
| Armenian hamster anti mouse CD183 (CXCR3), BUV395 | BD Biosciences | Cat #: 745689; Clone: CXCR3-173; RRID: AB_2743174; dilution: 1 to 100 |
| Mouse anti mouse Ly-108 (SlamF6), BB700 | BD Biosciences | Cat #: 742272; Clone: 13G3; dilution: 1 to 400 |
| Armenian hamster anti CD196 (CCR6), PE/Dazzle 594 | Biolegend | Cat #: 129822; Clone: 29-2L17; RRID: AB_2687019; dilution: 1 to 200 |
| Chemicals, peptides, and recombinant proteins | ||
| SIINFEKL-Kb monomer | NIH Tetramer Core | |
| SIINFEKL peptide | Genscript | |
| Critical commercial assays | ||
| Thru-Plex-FD Library Prep Kit | Rubicon Genomics | |
| Nextera XT Library Prep Kit | Illumina | FC-131-1096 |
| Chromium Next GEM Single Cell 5′ Library and Gel Bead Kit v1.1 | 10X Genomics | 1000167 |
| Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell | 10X Genomics | 1000071 |
| Deposited data | ||
| 10X and SmartSeq2 Single Cell RNA-seq | This Paper | GSE182028 |
| Sade-Feldman et al SmartSeq2 Single Cell RNA-seq | Sade-Feldman et al., 2018 | GSE120575 |
| Tirosh et al SmartSeq2 Single Cell RNA-seq | Tirosh et al., 2016 | GSE89567 |
| Experimental models: Organisms/strains | ||
| Mouse: B6.129S4-Krastm4Tyj/J | Jackson Laboratory | RRID: IMSR_JAX:008179 |
| Mouse: B6.129P2-Trp53tm1Brn/J | Jackson Laboratory | RRID: IMSR_JAX:008462 |
| Recombinant DNA | ||
| Plasmid: Lenti-LucOS | Addgene | Addgene_22777 |
| Software and algorithms | ||
| RSEM v1.2.8 | Li and Dewey, 2011 | http://bmcbioinformatbmc.biomedcentral.coc/articles/10.1186/1147-2105-12-323 |
| Prism | GraphPad | |
| Original Code | This Paper | https://github.com/RebeccaHerbst/TCF7_Tumor_Specific_CD8_T_cells |