Fig. 2.

CD200AR-L upregulated DAP10 and DAP12 pathways. Preliminary nanostring analysis suggest that the CD200AR-L activated the DAP10 and DAP 12 pathways. To validate these results, CD11b cells isolated from wild-type mice were pulsed with the CD200AR-L, cells were harvested at various times analyzed for A transcription levels and B, C Western analysis for protein levels. In separate experiments, D-H cells were harvested at 6 and 18 h; select signaling molecules were analyzed for transcription levels. I To profile phosphorylation, cells were pulsed for 5 min and analyzed by Fe-IMAC enrichment and LC-MS/MS to derive a Sigmoidal plot of quantitative changes (log2) between CD200AR-L treatment and control. Error bars represent standard deviation, *p < 0.05, **p < 0.005, ***p < 0.0005 by t test