Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Mycobacterium tuberculosis) | qcrC | Mycobrowser | Rv2194 | https://mycobrowser.epfl.ch/genes/Rv2194 |
Gene (M. tuberculosis) | qcrA | Mycobrowser | Rv2195 | https://mycobrowser.epfl.ch/genes/Rv2195 |
Gene (M. tuberculosis) | qcrB | Mycobrowser | Rv2196 | https://mycobrowser.epfl.ch/genes/Rv2196 |
Strain, strain background (Mycobacterium smegmatis) | mc2 51 | Li et al., 2014 | ||
Genetic reagent (include species here) | pVV16-QcrCAB-His10 | This paper | Construct contains the qcrCAB operon encoding three subunits | |
Commercial assay or kit | ClonExpress II One Step Cloning Kit | Vazyme | C112-01 | |
Chemical compound, drug | Q203 | MCE | HY-101040 | Prepare stock solution in DMSO |
Chemical compound, drug | TB47 | Lu et al., 2019 | Prepare stock solution in DMSO | |
Chemical compound, drug | LMNG | Anatrace | NG310 | |
Chemical compound, drug | Digitonin | BIOSYNTH | D-3200 | |
Software, algorithm | SerialEM | Mastronarde, 2003 | Version 3.6 | |
Software, algorithm | MotionCor2 | Zheng et al., 2017 | Version 1.2.1 | |
Software, algorithm | RELION | Zivanov et al., 2019 | Version 3.03 | |
Software, algorithm | cryoSPARC | Punjani et al., 2017 | Version 3.2.0 | |
Software, algorithm | Phyre2 | Kelley et al., 2015 | Version 2.0 | |
Software, algorithm | UCSF Chimera | Pettersen et al., 2004 | Version 1.12 | |
Software, algorithm | COOT | Emsley et al., 2010 | Version 0.8.9 | |
Software, algorithm | PHENIX | Adams et al., 2010 | Version 1.16 | |
Software, algorithm | PyMOL | Schrödinger LLC, 2017 | Version 2.0 | |
Software, algorithm | ChemDraw | Li et al., 2004 | Version 19.0 |