Table 1.
Currently available pre-made CRISPR libraries.
| Type of Screen | Library Name | AddGene Catalog Number |
Genes Targeted |
gRNAs Per Gene |
Total gRNAs |
|---|---|---|---|---|---|
| Genome-Wide Libraries | |||||
| Ko | GeCKO v2 | 1000000048 and 1000000049 |
19,052 | 6 | 123,411 |
| Ko | Toronto v3 | 90294 and 125517 |
18,053 | 4 | 70,948 |
| Ko | Brunello | 73179 and 73178 |
19,114 | 4 | 76,441 |
| Ko | Gattinara | 136986 | 19,993 | 2 | 40,964 |
| Ko | Mini-Human AsCas12a-Based Library | 130630 | 16,997 | 3–4 | 17,032 arrays (3–4 gRNAs per array) |
| Ko | BARBEKO (for screening with a base editor) | 174163 | 17,501 | 3 | 53,502 |
| CRISPRi | Dolcetto | 1000000114 | 18,901 (Set A); 18,899 (Set B) | 3–6 | 57,050 (Set A); 57,011 (Set B) |
| CRISPRi | CRISPRi-v2 | 83969 1000000090 |
18,905 | 5–10 | 104,535 209,070 |
| CRISPRa | Calabrese | 1000000111 | 18,885 (Set A); 18,843 (Set B) | 3–6 | 56,762 (Set A); 56,476 (Set B) |
| CRISPRa | CRISPRa-v2 | 83978 1000000091 |
18,915 | 5 or 10 | 104,540 or 209,080 |
| CRISPRa | SAM Library (3-plasmid system) | 1000000057 and 1000000074 |
23,430 | 3 | 70,290 |
| Pathway-Specific Libraries | |||||
| Ko | Human Interferon-Stimulated Gene CRISPR Knockout Library | 125753 | 1902 | 8 | 15,416 |
| Ko | Bison sgRNA Library (ubiquitination and deubiquitination genes) | 169942 | 713 | 4 | 2852 |
| Ko | Li Human UBDUB CRISPR Knockout Library (ubiquitination and deubiquitination genes) | 171531 | 1500 | ~6 | 9274 |
| CRISPRa | Wright Human Membrane Protein Activation Library (surface proteins) | 113345 | 6213 | 7–14 | 58,570 |