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. 2021 Oct 26;9(11):2226. doi: 10.3390/microorganisms9112226

Figure 1.

Figure 1

Schematic representation of tRNACys-PCR strategy for the B. cereus group. The cluster of 15 to 17 tRNA genes and the orientation of three specific genes located downstream of tRNALeu is shown. The primers indicated by the arrows were predicted to yield products of 1145 and 1430 bp, respectively (see Methodology). Phylogenetic analysis from these regions were obtained in MEGA X, using the neighbor-joining method. B. subtilis subsp. subtilis 168 was designated as the outgroup taxon.