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. 2021 Oct 26;9(11):2226. doi: 10.3390/microorganisms9112226

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative results for toxigenic characteristics. Panel (A) shows a representative electrophoresis gel for multiplex PCR. Lane M contains a Thermo Scientific GeneRuler 1 Kb DNA ladder, lane 1; strain SA5-1, lane 2; strain SA, lane 3; strain SB-2, lane 4, 5, and 6 is the control (B. cereus ATCC 10876) with single or multiplex PCR respectively. The arrows show the PCR amplification product for hblC (750 bp) and nheA (500 bp) toxin genes. Panel (B) shows a representative Duopath test for detection of toxin production indicated by a red line in both the test (Nhe and/or Hbl) and control zones (C). From left to right; B. cereus ATCC 10876 (1), SA75-1 (2), and SD20-1 (9).