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. 2021 Nov 1;13(11):1827. doi: 10.3390/pharmaceutics13111827

Figure 2.

Figure 2

Water consumption and urinary output in aspirin + Li-treated rats. Rats were fed regular food (control) or lithium-containing food [0.1% (LLD) or 0.2% (STD)] for 42 days. Low-dose aspirin (1 mg/kg, ip) was administered alone or together with Li. At indicated days, 24 h water consumption (a,b) and urinary output (c,d) were measured as described in Materials and Methods. Results are means ± SEM of a single representative experiment out of two demonstrating a similar pattern with 9–12 (day 21) or 6 (day 42) rats per group in the depicted experiment. (a) Water consumption day 21, two-way ANOVA: ASA effect, F1,59 = 7.938, p = 0.007; Li effect, F2,59 = 635.4, p < 0.0001; aspirin x Li interaction, F2,59 = 4.091, p = 0.021. Post hoc Fisher’s LSD test: Control vs. LLD-Li; Control vs. STD-Li, p < 0.0001; LLD-Li vs. STD-Li, p < 0.0001; LLD-Li vs. LLD-Li + ASA, p = 0.34; STD-Li vs. STD-Li + ASA, p = 0.0001. (b) Water consumption day 42, two-way ANOVA: ASA effect, F1,30 = 2.689 × 10−5, p = 0.9959; Li effect, F2,30 = 320.5, p < 0.0001; aspirin X Li interaction: F2,30 = 0.069, p = 0.9328. Post hoc Fisher’s LSD test: Control vs. LLD-Li, p = 0.015; Control vs. STD-Li, p < 0.0001; LLD-Li vs. STD-Li, p < 0.0001; LLD-Li vs. LLD-Li + ASA, p = 0.77; STD-Li vs. STD-Li + ASA, p = 0.83. (c) Urinary output day 21, two-way ANOVA: ASA effect, F1,59 = 4.528, p = 0.037; Li effect: F2,59 = 729.5, p <0.0001; aspirin X Li interaction, F2,59 = 3.384, p = 0.04. Post hoc Fisher’s LSD test: Control vs. LLD-Li, p = 0.0002; Control vs. STD-Li, p < 0.0001; LLD-Li vs. STD-Li, p < 0.0001; LLD-Li vs. LLD-Li + ASA, p = 0.523; STD-Li vs. STD-Li + ASA, p = 0.0009. (d) Urinary output day 42, two-way ANOVA: ASA effect, F1,30 = 0.0044, p = 0.9475; Li effect, F2,30 = 241.2, p < 0.0001; aspirin X Li interaction, F2,30 = 0.07569, p = 0.9273. Post hoc Fisher’s LSD test: Control vs. LLD-Li, p < 0.0001; Control vs. STD-Li, p < 0.0001; LLD-Li vs. STD-Li, p < 0.0001; LLD-Li vs. LLD-Li + ASA, p = 0.80; STD-Li vs. STD-Li + ASA, p = 0.895. Asterisks and symbols denote the following: *—p < 0.05 vs. control; #—p < 0.05 vs. LLD-Li; ^—p < 0.05 vs. STD-Li; $—p < 0.05 vs. LLD-Li + ASA. Abbreviations: ASA—acetylsalicylic acid, LLD—low-low dose, Li—lithium, STD—standard dose.