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UV-vis spectra before (red line) and after (green line) purging with nitrogen gas HbNPs fabricated without and with the addition of trehalose (TRE) at a Hb:TRE ratio of 20:1 or 5:1. Several concentrations of Hb were considered to fabricate the HbNPs (i.e., 50, 75, and 100 mg mL−1 Hb). The HbNPs were prepared using 12.5 mg mL−1 PLGA and 1% PVA.
