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UV-vis spectra of oxygenated (oxy-Hb) and deoxygenated (deoxy-Hb) Hb after successively purging with compressed air (red lines) and nitrogen (N2) gas (green lines) the optimized HbNPs (i.e., HbNPs-26). The HbNPs were fabricated using 75 mg mL−1 Hb, 3 mg mL−1 PLGA, and 0.5% PVA.
