Table 1.
Production of HCA3 ligands in culture supernatants of Bifidobacterium strains.
| Concentration (μM) | |||||||
|---|---|---|---|---|---|---|---|
| Species | Isolation Source | Strain | OD600 | 4-OH-PLA | PLA | ILA | LeuA |
| Infant-type HRB | |||||||
| B. bifidum | Infant feces | ATCC29521T | 0.78 ± 0.03 | 14.5 ± 1.3 | 492.9 ± 127.5 | 19.0 ± 5.1 | 131.6 ± 8.2 |
| Infant feces | NITE BP-02429 | 0.84 ± 0.05 | 6.8 ± 0.6 | 184.5 ± 25.5 | 7.4 ± 1.9 | 26.9 ± 6.2 | |
| Infant feces | NITE BP-02431 | 1.10 ± 0.02 | 17.6 ± 1.8 | 248.3 ± 57.5 | 13.8 ± 3.6 | 60.8 ± 12.1 | |
| B. breve | Intestine of infant | ATCC15700T | 1.24 ± 0.03 | 4.2 ± 1.3 | 23.0 ± 3.6 | 10.0 ± 2.1 | 99.3 ± 6.6 |
| Infant feces | FERM BP-11175 (MCC 1274) | 1.22 ± 0.02 | 12.6 ± 2.5 | 26.1 ± 4.2 | 9.5 ± 2.3 | 48.8 ± 2.4 | |
| Infant feces | NITE BP-02622 (M-16V) | 1.33 ± 0.01 | 8.1 ± 2.0 | 33.3 ± 7.3 | 10.6 ± 2.7 | 122.6 ± 4.7 | |
| B. longum subsp. infantis | Intestine of infant | ATCC15697T | 1.35 ± 0.02 | 16.2 ± 3.7 | 63.3 ± 11.3 | 6.9 ± 2.0 | 44.1 ± 1.1 |
| Intestine of infant | NITE BP-02623 (M-63) | 1.34 ± 0.02 | 12.9 ± 2.7 | 72.3 ± 13.6 | 6.6 ± 1.9 | 63.2 ± 2.3 | |
| B. longum subsp. longum | Intestine of adult | ATCC15707T | 1.14 ± 0.01 | 8.9 ± 2.4 | 123.0 ± 20.8 | 23.6 ± 5.0 | 181.8 ± 18.1 |
| Infant feces | NITE BP-02621 (BB536) | 1.24 ± 0.03 | 23.6 ± 2.9 | 152.2 ± 11.4 | 10.2 ± 1.9 | 230.1 ± 38.7 | |
| Average of infant-type HRB | 1.16 ± 0.2 | 12.5 ± 5.7 | 141.9 ± 144.2 | 11.7 ± 5.6 | 78.2 ± 65.9 | ||
| Adult-type HRB | |||||||
| B. adolescentis | Intestine of adult | ATCC15703T | 1.20 ± 0.01 | 2.2 ± 1.2 | 18.2 ± 3.9 | 0.2 ± 0.1 | 46.1 ± 3.2 |
| B. angulatum | Feces, human | ATCC27535T | 1.18 ± 0.03 | 10.7 ± 1.0 | 72.4 ± 10.3 | 4.6 ± 1.7 | 255.6 ± 34.2 |
| B. dentium | Dental caries | DSM20436T | 1.14 ± 0.05 | 0.7 ± 0.7 | 9.9 ± 2.0 | 0.9 ± 0.3 | 112.7 ± 12.2 |
| B. pseudocatenulatum | Feces, human | ATCC27919T | 1.19 ± 0.04 | 0.6 ± 0.9 | 14.8 ± 4.2 | 0.8 ± 0.2 | 136.4 ± 14.9 |
| Average of adult-type HRB | 1.18 ± 0.02 | 3.6 ± 4.8 * | 28.8 ± 29.2 | 1.6 ± 2.0 ** | 137.7 ± 87.4 | ||
| Non-HRB | |||||||
| B. animalis subsp.lactis | yoghurt | DSM10140T | 1.03 ± 0.00 | 2.6 ± 1.0 | 16.6 ± 3.2 | 0.9 ± 0.1 | 60.1 ± 12.9 |
| B. animalis subsp. animalis | Rat feces | ATCC25527T | 1.05 ± 0.02 | 0.7 ± 0.5 | 18.6 ± 4.2 | 0.7 ± 0.2 | 103.0 ± 10.4 |
| B. pseudolongum subsp. globosum | Rumen | JCM5820T | 0.85 ± 0.02 | 1.5 ± 0.7 | 8.9 ± 2.8 | 1.0 ± 0.8 | 46.8 ± 2.3 |
| B. pseudolongum subsp. pseudolongum | Swine feces | ATCC25526T | 0.95 ± 0.01 | 4.6 ± 0.9 | 35.4 ± 3.9 | 1.3 ± 0.2 | 216.9 ± 36.8 |
| B. thermophilum | Swine feces | ATCC 25525T | 1.25 ± 0.03 | 0.6 ± 0.3 | 23.3 ± 3.9 | 1.3 ± 0.3 | 170.9 ± 29.3 |
| Average of non-HRB | 1.03 ± 0.15 | 1.98 ± 1.69 ** | 20.53 ± 9.78 | 1.04 ± 0.27 *** | 119.55 ± 72.85 | ||
Concentrations and culture growth (OD600) are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, significant difference vs. infant-type HRB.