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. 2021 Nov 7;13(11):3968. doi: 10.3390/nu13113968

Figure 4.

Figure 4

Grifola extract (GE) reduces C. elegans fat accumulation in a glucose-loaded medium through daf-16 and daf-2 up-regulation. (A) Nile Red quantification of NGM-control and GE (20 µg/mL)-treated worms grown in a medium supplemented with glucose (10 mM). Orlistat (6 µg/mL) was used as the positive control. The results are expressed as the mean ± SEM relative to untreated control worms. (B) Expression levels of skn-1, daf-16, and daf-2 genes in GE-treated worms in comparison with the untreated control worms grown in a medium with glucose overload. Results are expressed as the fold-difference expression levels of each gene in GE-treated worms compared with the control, calculated with the 2−∆∆Ct method. (C) Nile Red quantification of NGM control and GE (20 µg/mL)-treated worms for skn-1 and daf-16 mutants. (D) Nile Red quantification of NGM control and GE (20 µg/mL)-treated daf-16 mutant worms grown in a medium supplemented with glucose. Significance refers to the effect of the treatments with respect to NGM-control worms (* p < 0.05; *** p < 0.001).