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. 2021 Nov 4;13(11):1859. doi: 10.3390/pharmaceutics13111859

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Poly(methyl methacrylate)/dioctadecyl dimethyl ammonium bromide (PMMA/DODAB) NPs at 0.5 mg/mL PMMA over a range of ovalbumin (OVA) concentrations: (a) photos of the dispersions; (b) OVA adsorption on NPs as a function of added OVA; (c) mean hydrodynamic diameter (Dz); (d) zeta-potential (ζ); (e) polydispersity of the dispersions (P). The stock solution of OVA used was 10 mg/mL whereas the red dot indicates properties of dispersions obtained from a 1 mg/mL OVA stock solution. The immunology tests were performed using the last stock solution of OVA. The red rectangle corresponds to the OVA concentration region of poor colloidal stability, meaning that DLS measurments could not be performed due to precipitation in the tubes.