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Production IL-4 (a), IL-10 (b), IL-2 (c) and IFN-γ (d) (pg/mL) elicited by cultured spleen cells obtained from naïve or animals immunized with ovalbumin (OVA), Al(OH)3/OVA, PMMA/DODAB/OVA (NPs/OVA). p < 0.05 compared to the naive group (•), p < 0.05 compared to the OVA group (#), p < 0.05 compared to the Al(OH)3/OVA group (∆). Splenocytes were challenged with OVA, medium (negative control) and concanavalin-A (Con-A) (positive control).
