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Effect of novel triterpenoids on (a) IRS-1 mRNA; (b) p-IRS-1 protein and expression in skeletal muscle of STZ-induced diabetic rats compared with the effect of insulin. The expression of mRNA and proteins was assessed by real-time PCR and Western blotting. β-actin was used as a loading control. Each bar represents mean ± SEM of 3 observations representing 6 animals. Significance at p < 0.05; *, compared with control, **, compared with diabetic control.
