Figure 4.

Doxycycline (doxy) abrogates the degradative and differentiation potential of hEDS-CM. IF analyses of FN-, COLLI-, and TNs-ECM organization, αvβ3 integrin and Snail1/Slug transcription factor expression, and α-SMA cytoskeleton assembly in control dermal fibroblasts grown in the presence of a pool of CM, recovered from six 72 h-grown control (C + C-CM) and six hEDS (C + hEDS-CM) cell strains, supplemented with 50 µM of doxy. Images are representative of three independent experiments. Scale bar: 12 μm.