Figure 7.

Crystal structure of (A) 1a, (C) 1c, and (E) 1h in complex with murine eIF4E. Key residues involved in cap analog binding are shown as sticks. Hydrogen bonds are shown as black dotted lines, while polar contacts are marked in red dotted lines. The structural water molecules are indicated as red non-bonded spheres only in the cave formed by Asp90, Ser92, Asn155, Pro100, Trp 102 and Trp 56 for clarity. (B–F) Overlay of three solved structures of eIF4E (yellow carbon) in complex with 1a (rose carbon), 1c (teal carbon) and 1h (cyan carbon), with the eIF4E (grey carbon) complex with m⁷GpppG (grey carbon) (PDB id: 1L8B, [29]), respectively. Relevant residues are shown in sticks, where dark blue is nitrogen, red oxygen, orange phosphate and light green chlorine.