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. 2021 Oct 28;13(11):2178. doi: 10.3390/v13112178

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Time-course of MDM infected with the SARS-CoV-2 strains/variant. The upper panel shows the Italian strain time-course. Cells are stained with the anti-N and anti-Spike protein (green fluorescence) and with DAPI (blue fluorescence), at 40× magnification in confocal microscopy. The lower panel shows the anti-N immunostaining (brown stain) of infected MDM (A,B) and the comparison of “blackberry-shaped” formations in the Chinese-derived (C) and PV10734 strains (D) 8 h p.i. with confocal microscopy. The area squared in (A) is enlarged in panel (B) with multinucleated MDM (red arrows) and individual cells (black arrows).